Vesicular trafficking plays a role in centriole disengagement and duplication
Author
Xie, ShuweiReinecke, James B
Farmer, Trey
Bahl, Kriti
Yeow, Ivana
Nichols, Benjamin J
McLamarrah, Tiffany A
Naslavsky, Naava
Rogers, Gregory C
Caplan, Steve
Affiliation
Univ Arizona, Canc Ctr, Dept Cellular & Mol MedIssue Date
2018-11-01
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AMER SOC CELL BIOLOGYCitation
Xie, S., Reinecke, J. B., Farmer, T., Bahl, K., Yeow, I., Nichols, B. J., ... & Caplan, S. (2018). Vesicular trafficking plays a role in centriole disengagement and duplication. Molecular biology of the cell, 29(22), 2622-2631.Journal
MOLECULAR BIOLOGY OF THE CELLRights
© 2018 Xie, Reinecke, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Centrosomes are the major microtubule-nucleating and microtubule-organizing centers of cells and play crucial roles in microtubule anchoring, organelle positioning, and ciliogenesis. At the centrosome core lies a tightly associated or "engaged" mother-daughter centriole pair. During mitotic exit, removal of centrosomal proteins pericentrin and Cep215 promotes "disengagement" by the dissolution of intercentriolar linkers, ensuring a single centriole duplication event per cell cycle. Herein, we explore a new mechanism involving vesicular trafficking for the removal of centrosomal Cep215. Using small interfering RNA and CRISPR/ Cas9 gene-edited cells, we show that the endocytic protein EHD1 regulates Cep215 transport from centrosomes to the spindle midbody, thus facilitating disengagement and duplication. We demonstrate that EHD1 and Cep215 interact and show that Cep215 displays increased localization to vesicles containing EHD1 during mitosis. Moreover, Cep215-containing vesicles are positive for internalized transferrin, demonstrating their endocytic origin. Thus, we describe a novel relationship between endocytic trafficking and the centrosome cycle, whereby vesicles of endocytic origin are used to remove key regulatory proteins from centrosomes to control centriole duplication.ISSN
1939-4586PubMed ID
30188792Version
Final published versionSponsors
National Institute of General Medical Sciences (NIGMS) [R01GM074876, P30GM106397]; National Cancer Institute [P30 CA23074]; NIH/NIGMS [R01GFM110166, R01GM126035]Additional Links
https://www.molbiolcell.org/doi/10.1091/mbc.E18-04-0241ae974a485f413a2113503eed53cd6c53
10.1091/mbc.E18-04-0241
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Except where otherwise noted, this item's license is described as © 2018 Xie, Reinecke, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
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