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dc.contributor.advisorLimesand, Sean
dc.contributor.authorOgunbunmi, Elizabeth Titlayo
dc.creatorOgunbunmi, Elizabeth Titlayo
dc.date.accessioned2019-06-13T03:51:12Z
dc.date.available2019-06-13T03:51:12Z
dc.date.issued2019
dc.identifier.citationOgunbunmi, Elizabeth Titlayo. (2019). Effects Of IGFs On Beta-Cell Proliferation In Ovine Models Exposed To Intrauterine Growth Restriction (Bachelor's thesis, University of Arizona, Tucson, USA).
dc.identifier.urihttp://hdl.handle.net/10150/632700
dc.description.abstractAn intrauterine growth restricted fetus refers to a fetus that has failed to reach its genetic growth potential due to maternal or placental limitations that lower the supply of oxygen and nutrients (Barry & Anthony, 2008). The fetus is sensitive to nutrient deficiencies and responds by altering organ growth and development, specifically nutrient sensing tissues like insulin producing, pancreatic beta cells. Insulin-like growth factor-1 (IGF-1) modulates beta-cell proliferation, and insulin-like growth factor binding proteins (IGFBPs) regulate IGFs via transport and distribution (Duan & Xu, 2005). Previous reports have shown that IGF-1 is lower and IGFBP-2 is higher in islets from IUGR fetal sheep (Chen, Rozance, Hay Jr., & Limesand, 2012). We hypothesize that decreases in IGF-1 signaling lower beta-cell proliferation rates. We propose two possible mechanisms: IGFBP2 is up-regulated to activate IGF-1 or IGFBP2 to prevents IGF-1 activity. In the present study, we investigated the interaction of IGF-1 and IGFBP-2 on the regulation of beta cell proliferation in pancreatic islets of IUGR and control fetuses. Isolated islets were cultured in media containing IGF-1, IGFBP-2, IGF-1 + IGFBP-2, and T3 and compared to media without supplemented hormones. Proliferation was measured by incubating the islets with 5-Ethynyl-2'-deoxyuridine (EdU). After 48 h in culture the islets were prepared for histology. Beta cells were immunostained with guinea pig anti-porcine insulin polyclonal antibody, Edu with the Click-It assay to measure proliferation, and nuclei with DAPI. Fluorescent images were taken with a digital camera. A thorough understanding of beta-cell physiology prenatally can possibly predict the onset of diabetes in adult patients.
dc.language.isoen
dc.publisherThe University of Arizona.
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.titleEffects Of IGFs On Beta-Cell Proliferation In Ovine Models Exposed To Intrauterine Growth Restriction
dc.typetext
dc.typeElectronic Thesis
thesis.degree.grantorUniversity of Arizona
thesis.degree.disciplineHonors College
thesis.degree.disciplinePhysiology
thesis.degree.nameB.A.
refterms.dateFOA2019-06-13T03:51:12Z


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