Adenosine Receptor Agonists And CD73 Effects On Avm Cell Calcification Markers
PublisherThe University of Arizona.
AbstractHeart valve calcification is a serious health issue that requires invasive treatment to resolve and is caused by calcium deposits on the heart valve tissue. Previous studies have suggested that the nucleoside adenosine and the surface cell enzyme CD73 are involved in the mechanism that leads to heart valve calcification. By using various treatments on atrio-ventricular mouse valve (AVM) cells grown in micromass cultures, including addition of adenosine receptor agonists and addition of the cell surface enzyme CD73, this relationship was further investigated. Quantitative PCR analysis showed that cells treated with CD73 had lower concentrations of the calcification marker SPP1 compared to the control and a greater amount of extracellular adenosine. Additionally, it was found that the A1 adenosine receptor and the A2A adenosine receptor agonists have a protective effect and a degenerative effect, respectively. These preliminary results show that this area requires further study and may someday be used in a pharmacological treatment for heart valve calcification or coupled with bioprosthetic replacement valves.