Cyclin-D1 And MUC1 Mediated Control Of Breast Cancer Cell Quiescence
Author
Malecha, Lindsey CatherineIssue Date
2019Advisor
Schroeder, Joyce
Metadata
Show full item recordPublisher
The University of Arizona.Rights
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.Abstract
The overexpression of cyclin-D1 has been shown previously to be a factor in regulating a shallow state of quiescence in breast cancer cells. This is thought to be due to the increased trafficking of EGFR, a cyclin-D1 transcription cofactor, to the nucleus as a result of interaction with MUC1.¹’² This study sought to create an accurate in vitro model of quiescent behavior and its regulation mechanism that can then be manipulated to find ways to modulate quiescence. In collaboration with Léa Boden, a model was identified for MDA-MB-468 triple negative breast cancer cells³. In MDA-MB-468 cells, shMUC1 variants had lower levels of cyclin-D1 expression and lower levels of chromatin-bound EGFR than the shCTL variants, thus supporting that MUC1 interaction affects EGFR trafficking to the nucleus.Type
textElectronic Thesis
Degree Name
B.S.Degree Program
Honors CollegeBiology