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dc.contributor.authorMlera, Luwanika
dc.contributor.authorBloom, Marshall E
dc.date.accessioned2019-06-26T19:54:04Z
dc.date.available2019-06-26T19:54:04Z
dc.date.issued2019-01-09
dc.identifier.citationMlera L, Bloom ME. Differential Zika Virus Infection of Testicular Cell Lines. Viruses. 2019; 11(1):42.en_US
dc.identifier.issn1999-4915
dc.identifier.pmid30634400
dc.identifier.doi10.3390/v11010042
dc.identifier.urihttp://hdl.handle.net/10150/633030
dc.description.abstractBackground: Zika virus is a mosquito-borne flavivirus responsible for recent outbreaks of epidemic proportions in Latin America. Sexual transmission of the virus has been reported in 13 countries and may be an important route of infection. Sexual transmission of ZIKV has mostly been male-to-female, and persistence of viral RNA in semen for up to 370 days has been recorded. The susceptibility to ZIKV of different testicular cell types merits investigation. Methods: We infected primary Sertoli cells, a primary testicular fibroblast Hs1.Tes, and 2 seminoma cell lines SEM-1 and TCam-2 cells with ZIKV Paraiba and the prototype ZIKV MR766 to evaluate their susceptibility and to look for viral persistence. A human neuroblastoma cell line SK-N-SH served as a control cell type. Results: Both virus strains were able to replicate in all cell lines tested, but ZIKV MR766 attained higher titers. Initiation of viral persistence by ZIKV Paraiba was observed in Sertoli, Hs1.Tes, SEM-1 and TCam-2 cells, but was of limited duration due to delayed cell death. ZIKV MR766 persisted only in Hs1.Tes and Sertoli cells, and persistence was also limited. In contrast, SK-N-SH cells were killed by both ZIKV MR766 and ZIKV Paraiba and persistence could not be established in these cells. Conclusions: ZIKV prototype strain MR766 and the clinically relevant Paraiba strain replicated in several testicular cell types. Persistence of ZIKV MR766 was only observed in Hs1.Tes and Sertoli cells, but the persistence did not last more than 3 or 4 passages, respectively. ZIKV Paraiba persisted in TCam-2, Hs1.Tes, Sertoli and SEM-1 cells for up to 5 passages, depending on cell type. TCam-2 cells appeared to clear persistent infection by ZIKV Paraiba.en_US
dc.description.sponsorshipDivision of Intramural Research program of the National Institute of Allergy and Infectious Diseases at the National Institutes of Healthen_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.urlhttps://www.mdpi.com/1999-4915/11/1/42en_US
dc.rights© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectZika virusen_US
dc.subjectflavivirusen_US
dc.subjecttestesen_US
dc.subjecttesticular cellsen_US
dc.subjectviral persistenceen_US
dc.titleDifferential Zika Virus Infection of Testicular Cell Linesen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, BIO5 Insten_US
dc.identifier.journalVIRUSES-BASELen_US
dc.description.noteOpen Access Journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleViruses
refterms.dateFOA2019-06-26T19:54:04Z


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