Surface Modification of Electrospun Scaffolds for Endothelialization of Tissue-Engineered Vascular Grafts Using Human Cord Blood-Derived Endothelial Cells
Author
Ardila, Diana CatalinaLiou, Jr-Jiun
Maestas, David
Slepian, Marvin J
Badowski, Michael
Wagner, William R.
Harris, David
Vande Geest, Jonathan P
Affiliation
Univ Arizona, Arizona Ctr Accelerated BioMed InnovatUniv Arizona, Inst Biocollaborat Res BIO5
Univ Arizona, Sarver Heart Ctr
Univ Arizona, Intervent Cardiol
Univ Arizona, Arizona Hlth Sci Ctr Biorepository
Univ Arizona, Arizona Hlth Sci Ctr Biorepository, Dept Immunobiol
Issue Date
2019-02-04Keywords
Vascular tissue engineeringendothelialization
human cord blood-derived endothelial cells
umbilical cord blood
vascular graft
Metadata
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MDPICitation
Ardila, D. C., Liou Jr, J., Maestas, D., Slepian, M. J., Badowski, M., Wagner, W., ... & Vande Geest, J. P. (2019). Surface Modification of Electrospun Scaffolds for Endothelialization of Tissue-Engineered Vascular Grafts Using Human Cord Blood-Derived Endothelial Cells. Journal of clinical medicine, 8(2), 185.Journal
JOURNAL OF CLINICAL MEDICINERights
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Tissue engineering has gained attention as an alternative approach for developing small diameter tissue-engineered vascular grafts intended for bypass surgery, as an option to treat coronary heart disease. To promote the formation of a healthy endothelial cell monolayer in the lumen of the graft, polycaprolactone/gelatin/fibrinogen scaffolds were developed, and the surface was modified using thermoforming and coating with collagen IV and fibronectin. Human cord blood-derived endothelial cells (hCB-ECs) were seeded onto the scaffolds and the important characteristics of a healthy endothelial cell layer were evaluated under static conditions using human umbilical vein endothelial cells as a control. We found that polycaprolactone/gelatin/fibrinogen scaffolds that were thermoformed and coated are the most suitable for endothelial cell growth. hCB-ECs can proliferate, produce endothelial nitric oxide synthase, respond to interleukin 1 beta, and reduce platelet deposition.Note
Open access journalISSN
2077-0383PubMed ID
30720769Version
Final published versionSponsors
NIH [1R56HL136517-01]; Cellular Approaches to Tissue Engineering and Regeneration training grant (CATER) at the University of Pittsburgh [5T32EB001026]; NSF [1337371]Additional Links
https://www.mdpi.com/2077-0383/8/2/185/htmae974a485f413a2113503eed53cd6c53
10.3390/jcm8020185
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Except where otherwise noted, this item's license is described as © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
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