Bacterial Rhizoplane Colonization Patterns of Buchloe dactyloides Growing in Metalliferous Mine Tailings Reflect Plant Status and Biogeochemical Conditions
AuthorHoneker, Linnea K
Neilson, Julia W
Root, Robert A
Maier, Raina M
AffiliationUniv Arizona, Dept Soil Water & Environm Sci
Keywords16S rRNA gene amplicon sequencing
MetadataShow full item record
CitationHoneker, L.K., Neilson, J.W., Root, R.A. et al. Microb Ecol (2017) 74: 853. https://doi.org/10.1007/s00248-017-0998-7
Rights© Springer Science+Business Media New York 2017.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractPlant establishment during phytostabilization of legacy mine tailings in semiarid regions is challenging due to low pH, low organic carbon, low nutrients, and high toxic metal(loid) concentrations. Plant-associated bacterial communities are particularly important under these harsh conditions because of their beneficial services to plants. We hypothesize that bacterial colonization profiles on rhizoplane surfaces reflect deterministic processes that are governed by plant health and the root environment. The aim of this study was to identify associations between bacterial colonization patterns on buffalo grass (Buchloe dactyloides) rhizoplanes and both plant status (leaf chlorophyll and plant cover) and substrate biogeochemistry (pH, electrical conductivity, total organic carbon, total nitrogen, and rhizosphere microbial community). Buffalo grass plants from mesocosm- and field-scale phytostabilization trials conducted with tailings from the Iron King Mine and Humboldt Smelter Superfund Site in Dewey-Humboldt, Arizona, were analyzed. These tailings are extremely acidic and have arsenic and lead concentrations of 2-4 g kg-1 substrate. Bacterial communities on rhizoplanes and in rhizosphere-associated substrate were characterized using fluorescence in situ hybridization and 16S rRNA gene amplicon sequencing, respectively. The results indicated that the metabolic status of rhizoplane bacterial colonizers is significantly related to plant health. Principal component analysis revealed that root-surface Alphaproteobacteria relative abundance was associated most strongly with substrate pH and Gammaproteobacteria relative abundance associated strongly with substrate pH and plant cover. These factors also affected the phylogenetic profiles of the associated rhizosphere communities. In summary, rhizoplane bacterial colonization patterns are plant specific and influenced by plant status and rhizosphere biogeochemical conditions.
Note12 month embargo; published online: 2 June 2017
VersionFinal accepted manuscript
SponsorsNational Institute of Environmental and Health Sciences (NIEHS) Superfund Research Program (SRP) [P42 ES004940, R01 ES01709]; National Science Foundation Graduate Research Fellowhip Program (NSF GRFP) [DGE-1143953]
- Effect of Re-acidification on Buffalo Grass Rhizosphere and Bulk Microbial Communities During Phytostabilization of Metalliferous Mine Tailings.
- Authors: Honeker LK, Gullo CF, Neilson JW, Chorover J, Maier RM
- Issue date: 2019
- Abundance and Activity of 16S rRNA, AmoA and NifH Bacterial Genes During Assisted Phytostabilization of Mine Tailings.
- Authors: Nelson KN, Neilson JW, Root RA, Chorover J, Maier RM
- Issue date: 2015
- Resolving colocalization of bacteria and metal(loid)s on plant root surfaces by combining fluorescence in situ hybridization (FISH) with multiple-energy micro-focused X-ray fluorescence (ME μXRF).
- Authors: Honeker LK, Root RA, Chorover J, Maier RM
- Issue date: 2016 Dec
- Restoration with pioneer plants changes soil properties and remodels the diversity and structure of bacterial communities in rhizosphere and bulk soil of copper mine tailings in Jiangxi Province, China.
- Authors: Sun X, Zhou Y, Tan Y, Wu Z, Lu P, Zhang G, Yu F
- Issue date: 2018 Aug
- Phytostabilization of mine tailings using compost-assisted direct planting: Translating greenhouse results to the field.
- Authors: Gil-Loaiza J, White SA, Root RA, Solís-Dominguez FA, Hammond CM, Chorover J, Maier RM
- Issue date: 2016 Sep 15