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dc.contributor.advisorStamer, W. Daniel
dc.contributor.authorAshpole, Nicole
dc.creatorAshpole, Nicole
dc.date.accessioned2019-07-06T02:19:53Z
dc.date.available2019-07-06T02:19:53Z
dc.date.issued2012
dc.identifier.urihttp://hdl.handle.net/10150/633299
dc.description.abstractNitric Oxide (NO) is a radical produced by endothelial NO synthase (eNOS), which is regulated by shear stress in vascular endothelia. In humans, shear stress levels in Schlemm's Canal (SC) are calculated to be comparable to that of arteries, particularly at elevated intraocular pressure (IOP), a risk factor for glaucoma. To test if NO is part of an IOP regulatory loop, we investigated the relationship between NO and shear stress in SC cells. Cells were seeded into lbidi flow chambers and assayed for effects of continuous shear on cell alignment and NO production. Human umbilical vascular endothelial cells (HUVECS) were used as a positive control. Like HUVECS, SC cells aligned with the direction of flow. NO synthesis in both cell types doubled with an increase in shear from 0.1 to 10.0 dynes/cm2, suggesting that shear regulates NO production in SC cells and consequently may play a role in IOP regulation.
dc.language.isoen
dc.publisherThe University of Arizona.
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction, presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
dc.titleShear Stress Effects on Schlemm's Canal Cells
dc.typetext
dc.typeThesis-Reproduction (electronic)
thesis.degree.grantorUniversity of Arizona
thesis.degree.levelmasters
dc.contributor.committeememberStamer, W. Daniel
dc.contributor.committeememberVandeGeest, Jonathan
dc.contributor.committeememberLynch, Ronald
dc.contributor.committeememberSchwiegerling, Jim
thesis.degree.disciplineBiomedical Engineering
thesis.degree.nameM.S.
dc.description.noteDigitized from a paper copy provided by the Physiological Sciences Graduate Interdisciplinary Program.
refterms.dateFOA2019-07-06T02:19:53Z


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