Recombinant Microtubule End-Capping Proteins as Inorganic Surface Ligands
PublisherThe University of Arizona.
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AbstractMicrotubules are being investigated as organic templates for nanowire fabrication. Targeted immobilization of the plus and minus ends of the microtubule can be achieved using recombinant end tracking microtubule proteins. This work examines the effects of five recombinant microtubule proteins on microtubule polymerization to determine their usefulness as inorganic surface ligands. GST-GAMMAtubulin was found to retain its microtubule nucleating capacity and anchor microtubules to an inorganic surface. GST-CLIP-170-H2, HISCLIP- 170-H2, GST-P150glued, and GST-EBl were all found to retain their microtubule binding capacity. GST-P150glued and GST-EBl were used to immobilize microtubules to inorganic surfaces. Their activity was enhanced by the presence of the H2 fragments.