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    Transcription Profiling of Angiogensis using DNA Microarrays

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    Author
    Budoff, Adam
    Issue Date
    2000
    Advisor
    Hoying, James
    
    Metadata
    Show full item record
    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction, presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Abstract
    Angiogenesis is the process by which new blood vessels form from an existing vasculature. The degree of angiogenesis and the character of the resulting microvascular beds vary between different healing environments such as wound healing and tumor angiogenesis. These differences are due, in part, to qualitative and quantitative differences in the molecular function within the tissue undergoing angiogenesis. For this study, these two types of angiogenic environments were created in mice and characterized on a gene expression level by utilizing cDNA microarrays. A DNA microarray containing 58 mouse genes from many different molecular classes relevant to angiogenesis was manufactured and tested. Optimal conditions and protocols for the use of microarray technology were designed and implemented. Results show that a polymerinduced angiogenic wound healing response differs greatly in its transcription profile from a tumor, suggesting that different types of angiogenesis occur in different environments.
    Type
    text
    Thesis-Reproduction (electronic)
    Degree Name
    M.S.
    Degree Level
    masters
    Degree Program
    Biomedical Engineering
    Degree Grantor
    University of Arizona
    Collections
    Master's Theses

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