Show simple item record

dc.contributor.authorAL-Ghadban, Sara
dc.contributor.authorCromer, Walter
dc.contributor.authorAllen, Marisol
dc.contributor.authorUssery, Christopher
dc.contributor.authorBadowski, Michael
dc.contributor.authorHarris, David
dc.contributor.authorHerbst, Karen L.
dc.date.accessioned2019-07-10T18:19:14Z
dc.date.available2019-07-10T18:19:14Z
dc.date.issued2019
dc.identifier.citationSara AL-Ghadban, Walter Cromer, Marisol Allen, et al., “Dilated Blood and Lymphatic Microvessels, Angiogenesis, Increased Macrophages, and Adipocyte Hypertrophy in Lipedema Thigh Skin and Fat Tissue,” Journal of Obesity, vol. 2019, Article ID 8747461, 10 pages, 2019. https://doi.org/10.1155/2019/8747461.en_US
dc.identifier.issn2090-0708
dc.identifier.issn2090-0716
dc.identifier.doi10.1155/2019/8747461
dc.identifier.urihttp://hdl.handle.net/10150/633357
dc.description.abstractBackground and Aim. Lipedema is a common painful SAT disorder characterized by enlargement of fat primarily in the legs of women. Case reports of lipedema tissue samples demonstrate fluid and fibrosis in the interstitial matrix, increased macrophages, and adipocyte hypertrophy. The aims of this project are to investigate blood vasculature, immune cells, and structure of lipedema tissue in a cohort of women. Methods. Forty-nine participants, 19 controls and 30 with lipedema, were divided into groups based on body mass index (BMI): Non-Obese (BMI 20 to <30kg/m(2)) and Obese (BMI 30 to <40kg/m(2)). Histological sections from thigh skin and fat were stained with H&E. Adipocyte area and blood vessel size and number were quantified using ImageJ software. Markers for macrophages (CD68), mast cells (CD117), T cells (CD3), endothelial cells (CD31), blood (SMA), and lymphatic (D2-40 and Lyve-1) vessels were investigated by IHC and IF. Results. Non-Obese Lipedema adipocyte area was larger than Non-Obese Controls (p=0.005) and similar to Obese Lipedema and Obese Controls. Macrophage numbers were significantly increased in Non-Obese (p<0.005) and Obese (p<0.05) Lipedema skin and fat compared to Control groups. No differences in T lymphocytes or mast cells were observed when comparing Lipedema to Control in both groups. SMA staining revealed increased dermal vessels in Non-Obese Lipedema patients (p<0.001) compared to Non-Obese Controls. Lyve-1 and D2-40 staining showed a significant increase in lymphatic vessel area but not in number or perimeter in Obese Lipedema participants (p<0.05) compared to Controls (Obese and Non-Obese). Areas of angiogenesis were found in the fat in 30% of lipedema participants but not controls. Conclusion. Hypertrophic adipocytes, increased numbers of macrophages and blood vessels, and dilation of capillaries in thigh tissue of non-obese women with lipedema suggest inflammation, and angiogenesis occurs independent of obesity and demonstrates a role of altered vasculature in the manifestation of the disease.en_US
dc.description.sponsorshipLipedema Foundation; University of Arizona College of Medicine TREAT Programen_US
dc.language.isoenen_US
dc.publisherHINDAWI LTDen_US
dc.relation.urlhttps://www.hindawi.com/journals/jobe/2019/8747461/en_US
dc.rightsCopyright © 2019 Sara AL-Ghadban et al. This is an open access article distributed under the Creative Commons Attribution License.en_US
dc.titleDilated Blood and Lymphatic Microvessels, Angiogenesis, Increased Macrophages, and Adipocyte Hypertrophy in Lipedema Thigh Skin and Fat Tissueen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Coll Med, Dept Meden_US
dc.contributor.departmentUniv Arizona, Coll Med, TREAT Programen_US
dc.contributor.departmentUniv Arizona, Coll Med, Dept Immunobiol & Biorepositoryen_US
dc.identifier.journalJOURNAL OF OBESITYen_US
dc.description.noteOpen access journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleJournal of Obesity
dc.source.volume2019
dc.source.beginpage1
dc.source.endpage10
refterms.dateFOA2019-07-10T18:19:15Z


Files in this item

Thumbnail
Name:
8747461.pdf
Size:
4.586Mb
Format:
PDF
Description:
Final Published version

This item appears in the following Collection(s)

Show simple item record