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dc.contributor.authorNeerukonda, Sabari Nath
dc.contributor.authorTavlarides-Hontz, Phaedra
dc.contributor.authorMcCarthy, Fiona
dc.contributor.authorPendarvis, Kenneth
dc.contributor.authorParcells, Mark S
dc.date.accessioned2019-07-10T20:55:54Z
dc.date.available2019-07-10T20:55:54Z
dc.date.issued2019-02-05
dc.identifier.citationNeerukonda SN, Tavlarides-Hontz P, McCarthy F, Pendarvis K, Parcells MS. Comparison of the Transcriptomes and Proteomes of Serum Exosomes from Marek’s Disease Virus-Vaccinated and Protected and Lymphoma-Bearing Chickens. Genes. 2019; 10(2):116.en_US
dc.identifier.issn2073-4425
dc.identifier.pmid30764491
dc.identifier.doi10.3390/genes10020116
dc.identifier.urihttp://hdl.handle.net/10150/633361
dc.description.abstractMarek's disease virus (MDV) is the causative agent of Marek's disease (MD), a complex pathology of chickens characterized by paralysis, immunosuppression, and T-cell lymphomagenesis. MD is controlled in poultry production via vaccines administered in ovo or at hatch, and these confer protection against lymphoma formation, but not superinfection by MDV field strains. Despite vaccine-induced humoral and cell-mediated immune responses, mechanisms eliciting systemic protection remain unclear. Here we report the contents of serum exosomes to assess their possible roles as indicators of systemic immunity, and alternatively, tumor formation. We examined the RNA and protein content of serum exosomes from CVI988 (Rispens)-vaccinated and protected chickens (VEX), and unvaccinated tumor-bearing chickens (TEX), via deep-sequencing and mass spectrometry, respectively. Bioinformatic analyses of microRNAs (miRNAs) and predicted miRNA targets indicated a greater abundance of tumor suppressor miRNAs in VEX compared to TEX. Conversely, oncomiRs originating from cellular (miRs 106a-363) and MDV miRNA clusters were more abundant in TEX compared to VEX. Most notably, mRNAs mapping to the entire MDV genome were identified in VEX, while mRNAs mapping to the repeats flanking the unique long (IRL/TRL) were identified in TEX. These data suggest that long-term systemic vaccine-induced immune responses may be mediated at the level of VEX which transfer viral mRNAs to antigen presenting cells systemically. Proteomic analyses of these exosomes suggested potential biomarkers for VEX and TEX. These data provide important putative insight into MDV-mediated immune suppression and vaccine responses, as well as potential serum biomarkers for MD protection and susceptibility.en_US
dc.description.sponsorshipCollege of Agriculture and Natural Resources (CANR) of the University of Delaware; Avian Biosciences Center of the University of Delawareen_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.urlhttps://www.mdpi.com/2073-4425/10/2/116en_US
dc.rights© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectMarek’s diseaseen_US
dc.subjectacid labile subunit (IGFALS), COL22A1en_US
dc.subjectcancer-associated exosomesen_US
dc.subjectexosomesen_US
dc.subjectinsulin-like growth factoren_US
dc.subjectlymphomaen_US
dc.subjectvanin-1en_US
dc.titleComparison of the Transcriptomes and Proteomes of Serum Exosomes from Marek's Disease Virus-Vaccinated and Protected and Lymphoma-Bearing Chickensen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Dept Anim & Comparat Biomed Scien_US
dc.identifier.journalGENESen_US
dc.description.noteOpen access journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleGenes
refterms.dateFOA2019-07-10T20:55:55Z


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