Expression of SLC4A11 protein in mouse and rat medulla: a candidate transporter involved in outer medullary ammonia recycling
Final Published Version
AffiliationUniv Arizona, Dept Physiol, Banner Univ Med Ctr
MetadataShow full item record
CitationGee, M. T., Kurtz, I., & Pannabecker, T. L. (2019). Expression of SLC4A11 protein in mouse and rat medulla: a candidate transporter involved in outer medullary ammonia recycling. Physiological reports, 7(10).
Rights© 2019 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractSLC4A11 is a multifunctional membrane transporter involved with H+ transport, NH3 and alkaline pH stimulated H+ transport, and water transport. The role of SLC4A11 in the kidney is not well understood. A prior study has shown that in murine kidney, SLC4A11/LacZ staining is primarily in the long-looped descending thin limb (DTL) as determined by colocalization with aquaporin 1 (AQP1), a protein that is expressed in some, but not all, descending thin limb segments. Using a previously characterized polyclonal antibody, we demonstrate the selective expression of SLC4A11 in the upper DTLs (which are AQP1-positive) in the outer medulla and inner medulla with little or no expression in the lower DTLs (which are AQP-1-null). SLC4A11 also colocalized with AQP1 and the urea transporter UT-B in the mouse descending vasa recta, but was absent in mouse and rat ascending vasa recta. Mouse, but not rat, outer medullary collecting duct cells also labeled for SLC4A11. Our results are compatible with the hypothesis that in the inner stripe of the outer medulla, SLC4A11 plays a role in the countercurrent transport of ammonia absorbed from the outer medullary thick ascending limb and secreted into the long-looped DTLs. SLC4A11 can potentially modulate the rate of ammonia transport in the mouse outer medullary collecting duct. Our data suggest functionally unique SLC4A11 pathways in mouse and rat and complement previous studies of DTL Na+, urea and water permeability indicating that the upper and lower DTLs of long-looped nephrons are functionally distinct.
NoteOpen access journal
VersionFinal published version
SponsorsNational Institutes of Health: National Institute of Diabetes and Digestive and Kidney Diseases [DK083338]; Joint DMS/NIGMS Initiative under NSF [DMS-1263943]; NIH National Institute of Diabetes and Digestive and Kidney Diseases [DK077162]; Allan Smidt Charitable Fund; Factor Family Foundation; Ralph Block Family Foundation
Except where otherwise noted, this item's license is described as © 2019 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License.
- Descending thin limb of the intermediate loop expresses both aquaporin 1 and urea transporter A2 in the mouse kidney.
- Authors: Kim WY, Lee HW, Han KH, Nam SA, Choi A, Kim YK, Kim J
- Issue date: 2016 Jul
- Aquaporin-1 water channels in short and long loop descending thin limbs and in descending vasa recta in rat kidney.
- Authors: Nielsen S, Pallone T, Smith BL, Christensen EI, Agre P, Maunsbach AB
- Issue date: 1995 Jun
- Aquaporin-1 is not expressed in descending thin limbs of short-loop nephrons.
- Authors: Zhai XY, Fenton RA, Andreasen A, Thomsen JS, Christensen EI
- Issue date: 2007 Nov
- Architecture of kangaroo rat inner medulla: segmentation of descending thin limb of Henle's loop.
- Authors: Urity VB, Issaian T, Braun EJ, Dantzler WH, Pannabecker TL
- Issue date: 2012 Mar 15
- Alternative channels for urea in the inner medulla of the rat kidney.
- Authors: Nawata CM, Dantzler WH, Pannabecker TL
- Issue date: 2015 Dec 1