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dc.contributor.authorBeatty, Norman L
dc.contributor.authorBehrens-Bradley, Nicole
dc.contributor.authorLove, Maria
dc.contributor.authorMcCants, Finn
dc.contributor.authorSmith, Shannon
dc.contributor.authorSchmidt, Justin O
dc.contributor.authorHamer, Sarah A
dc.contributor.authorDorn, Patricia L
dc.contributor.authorAhmad, Nafees
dc.contributor.authorKlotz, Stephen A
dc.date.accessioned2019-07-26T23:37:45Z
dc.date.available2019-07-26T23:37:45Z
dc.date.issued2019
dc.identifier.citationBeatty, N. L., Behrens-Bradley, N., Love, M., McCants, F., Smith, S., Schmidt, J. O., ... & Klotz, S. A. (2019). Rapid detection of human blood in triatomines (kissing bugs) utilizing a lateral flow immunochromatographic assay-A pilot study. Memórias do Instituto Oswaldo Cruz, 114.en_US
dc.identifier.issn0074-0276
dc.identifier.pmid31166422
dc.identifier.doi10.1590/0074-02760190047
dc.identifier.urihttp://hdl.handle.net/10150/633543
dc.description.abstractBACKGROUND DNA- and proteomics-based techniques are currently used to identify a triatomine human blood meal. These methods are time consuming, require access to laboratories with sophisticated equipment, and trained personnel. OBJECTIVES We tested a rapid and specific immunochromatographic assay (that detects human blood in forensic samples) to determine if human blood was present in triatomines and their fecal excreta. METHODS We fed Triatoma rubida human blood (positive control) or mouse blood (negative control) and performed the assay on the abdominal contents and fecal excreta. Triatomine field specimens collected in and around human habitations and excreta were also tested. FINDINGS The assay was positive in triatomines fed human blood (N = 5/5) and fecal excreta from bugs known to have ingested human blood (N = 5/5). Bugs feeding on mice (N = 15/15) and their fecal excreta (N = 8/8) were negative for human blood. Human blood was detected in 47% (N = 23/49) triatomines, representing six different species, collected in the field. MAIN CONCLUSIONS The pilot study shows that this rapid and specific test may have applications in triatomine research. Further study is needed to determine the sensitivity of this assay compared to other well-established techniques, such as DNA- and proteomics-based methodologies and the assay's application in the field.en_US
dc.language.isoenen_US
dc.publisherFUNDACO OSWALDO CRUZen_US
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License. Copyright is held by the author(s) or the publisher. If your intended use exceeds the permitted uses specified by the license, contact the publisher for more information.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjecttriatomineen_US
dc.subjectkissing bugen_US
dc.subjecthuman blood detectionen_US
dc.subjectblood sourcesen_US
dc.subjectChagas diseaseen_US
dc.titleRapid detection of human blood in triatomines (kissing bugs) utilizing a lateral flow immunochromatographic assay - A pilot studyen_US
dc.typeArticleen_US
dc.identifier.eissn1678-8060
dc.contributor.departmentUniv Arizona, Coll Med, Dept Med, Div Infect Disen_US
dc.contributor.departmentUniv Arizona, Dept Immunobiol, Coll Meden_US
dc.identifier.journalMEMORIAS DO INSTITUTO OSWALDO CRUZen_US
dc.description.noteOpen access journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleMemorias do Instituto Oswaldo Cruz
refterms.dateFOA2019-07-26T23:37:46Z


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This is an open-access article distributed under the terms of the Creative Commons Attribution License. Copyright is held by the author(s) or the publisher. If your intended use exceeds the permitted uses specified by the license, contact the publisher for more information.
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License. Copyright is held by the author(s) or the publisher. If your intended use exceeds the permitted uses specified by the license, contact the publisher for more information.