Rapid detection of human blood in triatomines (kissing bugs) utilizing a lateral flow immunochromatographic assay - A pilot study
dc.contributor.author | Beatty, Norman L | |
dc.contributor.author | Behrens-Bradley, Nicole | |
dc.contributor.author | Love, Maria | |
dc.contributor.author | McCants, Finn | |
dc.contributor.author | Smith, Shannon | |
dc.contributor.author | Schmidt, Justin O | |
dc.contributor.author | Hamer, Sarah A | |
dc.contributor.author | Dorn, Patricia L | |
dc.contributor.author | Ahmad, Nafees | |
dc.contributor.author | Klotz, Stephen A | |
dc.date.accessioned | 2019-07-26T23:37:45Z | |
dc.date.available | 2019-07-26T23:37:45Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Beatty, N. L., Behrens-Bradley, N., Love, M., McCants, F., Smith, S., Schmidt, J. O., ... & Klotz, S. A. (2019). Rapid detection of human blood in triatomines (kissing bugs) utilizing a lateral flow immunochromatographic assay-A pilot study. Memórias do Instituto Oswaldo Cruz, 114. | en_US |
dc.identifier.issn | 0074-0276 | |
dc.identifier.pmid | 31166422 | |
dc.identifier.doi | 10.1590/0074-02760190047 | |
dc.identifier.uri | http://hdl.handle.net/10150/633543 | |
dc.description.abstract | BACKGROUND DNA- and proteomics-based techniques are currently used to identify a triatomine human blood meal. These methods are time consuming, require access to laboratories with sophisticated equipment, and trained personnel. OBJECTIVES We tested a rapid and specific immunochromatographic assay (that detects human blood in forensic samples) to determine if human blood was present in triatomines and their fecal excreta. METHODS We fed Triatoma rubida human blood (positive control) or mouse blood (negative control) and performed the assay on the abdominal contents and fecal excreta. Triatomine field specimens collected in and around human habitations and excreta were also tested. FINDINGS The assay was positive in triatomines fed human blood (N = 5/5) and fecal excreta from bugs known to have ingested human blood (N = 5/5). Bugs feeding on mice (N = 15/15) and their fecal excreta (N = 8/8) were negative for human blood. Human blood was detected in 47% (N = 23/49) triatomines, representing six different species, collected in the field. MAIN CONCLUSIONS The pilot study shows that this rapid and specific test may have applications in triatomine research. Further study is needed to determine the sensitivity of this assay compared to other well-established techniques, such as DNA- and proteomics-based methodologies and the assay's application in the field. | en_US |
dc.language.iso | en | en_US |
dc.publisher | FUNDACO OSWALDO CRUZ | en_US |
dc.rights | This is an open-access article distributed under the terms of the Creative Commons Attribution License. Copyright is held by the author(s) or the publisher. If your intended use exceeds the permitted uses specified by the license, contact the publisher for more information. | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | triatomine | en_US |
dc.subject | kissing bug | en_US |
dc.subject | human blood detection | en_US |
dc.subject | blood sources | en_US |
dc.subject | Chagas disease | en_US |
dc.title | Rapid detection of human blood in triatomines (kissing bugs) utilizing a lateral flow immunochromatographic assay - A pilot study | en_US |
dc.type | Article | en_US |
dc.identifier.eissn | 1678-8060 | |
dc.contributor.department | Univ Arizona, Coll Med, Dept Med, Div Infect Dis | en_US |
dc.contributor.department | Univ Arizona, Dept Immunobiol, Coll Med | en_US |
dc.identifier.journal | MEMORIAS DO INSTITUTO OSWALDO CRUZ | en_US |
dc.description.note | Open access journal | en_US |
dc.description.collectioninformation | This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu. | en_US |
dc.eprint.version | Final published version | en_US |
dc.source.journaltitle | Memorias do Instituto Oswaldo Cruz | |
refterms.dateFOA | 2019-07-26T23:37:46Z |