Genome-Wide CRISPR Screen Reveals Autophagy Disruption as the Convergence Mechanism That Regulates the NRF2 Transcription Factor
AffiliationUniv Arizona, Coll Pharm, Dept Pharmacol & Toxicol
Univ Arizona, Ctr Canc
MetadataShow full item record
PublisherAMER SOC MICROBIOLOGY
CitationKerins, M. J., Liu, P., Tian, W., Mannheim, W., Zhang, D. D., & Ooi, A. (2019). Genome-Wide CRISPR Screen Reveals Autophagy Disruption as the Convergence Mechanism That Regulates the NRF2 Transcription Factor. Molecular and cellular biology, 39(13), e00037-19.
JournalMOLECULAR AND CELLULAR BIOLOGY
Rights© 2019 American Society for Microbiology. All Rights Reserved.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractThe nuclear factor (erythroid 2)-like 2 (NRF2 or NFE2L2) transcription factor regulates the expression of many genes that are critical in maintaining cellular homeostasis. Its deregulation has been implicated in many diseases, including cancer and metabolic and neurodegenerative diseases. While several mechanisms by which NRF2 can be activated have gradually been identified over time, a more complete regulatory network of NRF2 is still lacking. Here we show through a genome-wide clustered regularly interspaced short palindromic repeat (CRISPR) screen that a total of 273 genes, when knocked out, will lead to sustained NRF2 activation. Pathway analysis revealed a significant overrepresentation of genes (18 of the 273 genes) involved in autophagy. Molecular validation of a subset of the enriched genes identified 8 high-confidence genes that negatively regulate NRF2 activity irrespective of cell type: ATG12, ATG7, GOSR1, IFT172, NRXN2, RAB6A, VPS37A, and the well-known negative regulator of NRF2, KEAP1 Of these, ATG12, ATG7, KEAP1, and VPS37A are known to be involved in autophagic processes. Our results present a comprehensive list of NRF2 negative regulators and reveal an intimate link between autophagy and NRF2 regulation.
Note6 month embargo; accepted manuscript posted online 22 April 2019
VersionFinal published version
SponsorsNational Institute of Environmental Health Sciences [R21ES027920]; National Science Foundation Graduate Research Fellowship Program [DGE-1746060]; [R01CA226920]; [R01DK109555]; [R01ES026845]; [P42ES004940]; [P30ES006694]
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