CFTR regulates B cell activation and lymphoid follicle development
Quintero, Joselyn Rojas
Vargas, Sara O
Patel, Avignat S
Owen, Caroline A
Gerard, Norma P
AffiliationUniv Arizona, Asthma & Airway Dis Res Ctr
MetadataShow full item record
CitationPolverino, F., Lu, B., Quintero, J. R., Vargas, S. O., Patel, A. S., Owen, C. A., ... & Cernadas, M. (2019). CFTR regulates B cell activation and lymphoid follicle development. Respiratory research, 20(1), 133.
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AbstractThe number of LFs, BAFF+, TLR4+ and proliferation marker Ki67+ B cells in lung explants or resections from subjects with CF and normal controls was quantified by immunostaining. The role of CFTR in B cell activation and LF development was then examined in two independent cohorts of uninfected CFTR-deficient mice (Cftr -/-) and wild type controls. The number of lung LFs, B cells and BAFF+, CXCR4+, immunoglobulin G+ B cells was examined by immunostaining. Lung and splenocyte B cell activation marker and major histocompatibility complex class II (MHC class II) expression was quantified by flow cytometry. Inflammatory cytokine levels were measured in supernatants from isolated B cells from Cftr -/- and wild type mice stimulated in vitro with Pseudomonas aeruginosa lipopolysaccharide (LPS).
NoteOpen access journal
VersionFinal published version
SponsorsCT/MA Cystic Fibrosis/Multiple Sclerosis Fund; May Family Trust; CF Foundation [DRUMMR0, DRUMMR1]