CFTR regulates B cell activation and lymphoid follicle development

Polverino, Francesca; Lu, Bao; Quintero, Joselyn Rojas; Vargas, Sara O; Patel, Avignat S; Owen, Caroline A; Gerard, Norma P; Gerard, Craig; Cernadas, Manuela

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Author

Polverino, Francesca
Lu, Bao
Quintero, Joselyn Rojas
Vargas, Sara O
Patel, Avignat S
Owen, Caroline A
Gerard, Norma P
Gerard, Craig
Cernadas, Manuela

Affiliation

Univ Arizona, Asthma & Airway Dis Res Ctr

Issue Date

2019-07-01

Citation

Polverino, F., Lu, B., Quintero, J. R., Vargas, S. O., Patel, A. S., Owen, C. A., ... & Cernadas, M. (2019). CFTR regulates B cell activation and lymphoid follicle development. Respiratory research, 20(1), 133.

Journal

RESPIRATORY RESEARCH

Rights

© The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.

Abstract

The number of LFs, BAFF+, TLR4+ and proliferation marker Ki67+ B cells in lung explants or resections from subjects with CF and normal controls was quantified by immunostaining. The role of CFTR in B cell activation and LF development was then examined in two independent cohorts of uninfected CFTR-deficient mice (Cftr -/-) and wild type controls. The number of lung LFs, B cells and BAFF+, CXCR4+, immunoglobulin G+ B cells was examined by immunostaining. Lung and splenocyte B cell activation marker and major histocompatibility complex class II (MHC class II) expression was quantified by flow cytometry. Inflammatory cytokine levels were measured in supernatants from isolated B cells from Cftr -/- and wild type mice stimulated in vitro with Pseudomonas aeruginosa lipopolysaccharide (LPS).

Note

Open access journal

ISSN

1465-993X

EISSN

1465-9921

PubMed ID

31262295

DOI

10.1186/s12931-019-1103-1

Version

Final published version

Collections

UA Faculty Publications