Single-Cell RNA Sequencing of Human Embryonic Stem Cell Differentiation Delineates Adverse Effects of Nicotine on Embryonic Development
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Univ Arizona, Dept Basic Med Sci, Coll Med, PhoenixIssue Date
2019-04-09Keywords
differentiationembryonic development
embryonic stem cells
nicotine
single-cell RNA sequencing
smoking
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CELL PRESSCitation
Guo, H., Tian, L., Zhang, J. Z., Kitani, T., Paik, D. T., Lee, W. H., & Wu, J. C. (2019). Single-Cell RNA Sequencing of Human Embryonic Stem Cell Differentiation Delineates Adverse Effects of Nicotine on Embryonic Development. Stem cell reports, 12(4), 772-786.Journal
STEM CELL REPORTSRights
© 2019 The Authors. This is an open access article under the CC BY-NC-ND license.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Nicotine, the main chemical constituent of tobacco, is highly detrimental to the developing fetus by increasing the risk of gestational complications and organ disorders. The effects of nicotine on human embryonic development and related mechanisms, however, remain poorly understood. Here, we performed single-cell RNA sequencing (scRNA-seq) of human embryonic stem cell (hESC)-derived embryoid body (EB) in the presence or absence of nicotine. Nicotine-induced lineage-specific responses and dysregulated cell-to-cell communication in EBs, shedding light on the adverse effects of nicotine on human embryonic development. In addition, nicotine reduced cell viability, increased reactive oxygen species (ROS), and altered cell cycling in EBs. Abnormal Ca2+ signaling was found in muscle cells upon nicotine exposure, as verified in hESC-derived cardiomyocytes. Consequently, our scRNA-seq data suggest direct adverse effects of nicotine on hESC differentiation at the single-cell level and offer a new method for evaluating drug and environmental toxicity on human embryonic development in utero.Note
Open access journalISSN
2213-6711PubMed ID
30827876Version
Final published versionSponsors
National Institutes of Health (NIH) [T32 EB009035, TRDRP 26FT-0029]; Burroughs Wellcome Fund [1015009, R01 HL130020, R01 HL113006, R01 HL141371, R01 HL132875, TRDRP 27IR-0012]ae974a485f413a2113503eed53cd6c53
10.1016/j.stemcr.2019.01.022
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Except where otherwise noted, this item's license is described as © 2019 The Authors. This is an open access article under the CC BY-NC-ND license.
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