Changes in the crystallographic structures of cardiac myosin filaments detected by polarization-dependent second harmonic generation microscopy
Borg, Thomas K.
Runyan, Raymond B.
Gao, Bruce Z.
AffiliationUniv Arizona, Dept Cell Biol & Anatonty
MetadataShow full item record
PublisherThe Optical Society
CitationYuan, C., Wang, Z., Borg, T. K., Ye, T., Baicu, C., Bradshaw, A., ... & Gao, B. Z. (2019). Changes in the crystallographic structures of cardiac myosin filaments detected by polarization-dependent second harmonic generation microscopy. Biomedical Optics Express, 10(7), 3183-3195.
JournalBIOMEDICAL OPTICS EXPRESS
RightsCopyright © 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractDetecting the structural changes caused by volume and pressure overload is critical to comprehending the mechanisms of physiologic and pathologic hypertrophy. This study explores the structural changes at the crystallographic level in myosin filaments in volume- and pressure-overloaded myocardia through polarization-dependent second harmonic generation microscopy. Here, for the first time, we report that the ratio of nonlinear susceptibility tensor components d33/d15 increased significantly in volume- and pressure-overloaded myocardial tissues compared with the ratio in normal mouse myocardial tissues. Through cell stretch experiments, we demonstrated that mechanical tension plays an important role in the increase of d33/d15 in volume- and pressure-overloaded myocardial tissues.
NoteOpen access journal
VersionFinal published version
SponsorsNational Institutes of Health through SC COBRE [P20RR021949, P20GM130451]; National Science Foundation EPSCoR Program [OIA-1655740]; National Natural Science Foundation of China ; [R01HL124782]; [R01HL144927]