Deciphering the complex interplay between microbiota, HPV, inflammation and cancer through cervicovaginal metabolic profiling
AuthorIlhan, Zehra Esra
Roe, Denise J
Chase, Dana M
Herbst-Kralovetz, Melissa M
AffiliationUniv Arizona, Coll Med Phoenix, Dept Obstet & Gynecol
Univ Arizona, Coll Med Phoenix, Dept Basic Med Sci
Univ Arizona, UA Canc Ctr
KeywordsAmino acid degradation
Cervical dysplasia and cancer
Lipids and nucleotides
MetadataShow full item record
PublisherELSEVIER SCIENCE BV
CitationIlhan, Z. E., Łaniewski, P., Thomas, N., Roe, D. J., Chase, D. M., & Herbst-Kralovetz, M. M. (2019). Deciphering the complex interplay between microbiota, HPV, inflammation and cancer through cervicovaginal metabolic profiling. EBioMedicine.
RightsCopyright © 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractBackground: Dysbiotic vaginal microbiota have been implicated as contributors to persistent HPV-mediated cervical carcinogenesis and genital inflammation with mechanisms unknown. Given that cancer is a metabolic disease, metabolic profiling of the cervicovaginal microenvironment has the potential to reveal the functional interplay between the host and microbes in HPV persistence and progression to cancer. Methods: Our study design included HPV-negative/positive controls, women with low-grade and high-grade cervical dysplasia, or cervical cancer (n = 78). Metabolic fingerprints were profiled using liquid chromatography-mass spectrometry. Vaginal microbiota and genital inflammation were analysed using 16S rRNA gene sequencing and immunoassays, respectively. We used an integrative bioinformatic pipeline to reveal host and microbe contributions to the metabolome and to comprehensively assess the link between HPV, microbiota, inflammation and cervical disease. Findings: Metabolic analysis yielded 475 metabolites with known identities. Unique metabolic fingerprints discriminated patient groups from healthy controls. Three-hydroxybutyrate, eicosenoate, and oleate/vaccenate discriminated (with excellent capacity) between cancer patients versus the healthy participants. Sphingolipids, plasmalogens, and linoleate positively correlated with genital inflammation. Non-Lactobacillus dominant communities, particularly in high-grade dysplasia, perturbed amino acid and nucleotide metabolisms. Adenosine and cytosine correlated positively with Lactobacillus abundance and negatively with genital inflammation. Glycochenodeoxycholate and carnitine metabolisms connected non-Lactobacillus dominance to genital inflammation. Interpretation: Cervicovaginal metabolic profiles were driven by cancer followed by genital inflammation, HPV infection, and vaginal microbiota. This study provides evidence for metabolite-driven complex host-microbe interactions as hallmarks of cervical cancer with future translational potential. (C) 2019 The Authors. Published by Elsevier B.V.
NoteOpen access journal
VersionFinal published version
SponsorsFlinn Foundation ; Banner Foundation in Obstetrics and Gynecology Research; National Institutes of Health NCI [P30 CA023074]
- The vaginal metabolome and microbiota of cervical HPV-positive and HPV-negative women: a cross-sectional analysis.
- Authors: Borgogna JC, Shardell MD, Santori EK, Nelson TM, Rath JM, Glover ED, Ravel J, Gravitt PE, Yeoman CJ, Brotman RM
- Issue date: 2020 Jan
- Features of the cervicovaginal microenvironment drive cancer biomarker signatures in patients across cervical carcinogenesis.
- Authors: Łaniewski P, Cui H, Roe DJ, Barnes D, Goulder A, Monk BJ, Greenspan DL, Chase DM, Herbst-Kralovetz MM
- Issue date: 2019 May 14
- The vaginal microbiota, human papillomavirus and cervical dysplasia: a systematic review and network meta-analysis.
- Authors: Norenhag J, Du J, Olovsson M, Verstraelen H, Engstrand L, Brusselaers N
- Issue date: 2020 Jan
- Human papillomavirus infection and cervical intraepithelial neoplasia progression are associated with increased vaginal microbiome diversity in a Chinese cohort.
- Authors: Chen Y, Qiu X, Wang W, Li D, Wu A, Hong Z, Di W, Qiu L
- Issue date: 2020 Aug 26
- Cervicovaginal microbiome and natural history of HPV in a longitudinal study.
- Authors: Usyk M, Zolnik CP, Castle PE, Porras C, Herrero R, Gradissimo A, Gonzalez P, Safaeian M, Schiffman M, Burk RD, Costa Rica HPV Vaccine Trial (CVT) Group.
- Issue date: 2020 Mar