Low-cost, high-speed near infrared reflectance confocal microscope
Author
Gong, ChengKulkarni, Nachiket
Zhu, Wenbin
Nguyen, Christopher David
Curiel-Lewandrowski, Clara
Kang, Dongkyun
Affiliation
Univ Arizona, Coll Opt SciUniv Arizona, Canc Ctr
Univ Arizona, Dept Biomed Engn
Univ Arizona, Bio5 Inst
Issue Date
2019-06-21
Metadata
Show full item recordPublisher
OPTICAL SOC AMERCitation
Gong, C., Kulkarni, N., Zhu, W., Nguyen, C. D., Curiel-Lewandrowski, C., & Kang, D. (2019). Low-cost, high-speed near infrared reflectance confocal microscope. Biomedical Optics Express, 10(7), 3497-3505.Journal
BIOMEDICAL OPTICS EXPRESSRights
Copyright © 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
We have developed a low-cost, near-infrared (NIR) reflectance confocal microscope (RCM) to overcome challenges in the imaging depth and speed found in our previously-reported smartphone confocal microscope. In the new NIR RCM device, we have used 840 nm superluminescent LED (sLED) to increase the tissue imaging depth and speed. A new confocal detection optics has been developed to maintain high lateral resolution even when a relatively large slit width was used. The material cost of the NIR RCM device was still low, similar to$5,200. The lateral resolution was 1.1 mu m and 1.3 mu m along the vertical and horizontal directions, respectively. Axial resolution was measured as 11.2 mu m. In vivo confocal images of human forearm skin obtained at the imaging speed of 203 frames/sec clearly visualized characteristic epidermal and dermal cellular features of the human skin. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing AgreementNote
Open access journalISSN
2156-7085Version
Final published versionSponsors
National Institutes of Health/Fogarty International Center [R21TW010221]ae974a485f413a2113503eed53cd6c53
10.1364/boe.10.003497