Low-cost, high-speed near infrared reflectance confocal microscope
Nguyen, Christopher David
AffiliationUniv Arizona, Coll Opt Sci
Univ Arizona, Canc Ctr
Univ Arizona, Dept Biomed Engn
Univ Arizona, Bio5 Inst
MetadataShow full item record
PublisherOPTICAL SOC AMER
CitationGong, C., Kulkarni, N., Zhu, W., Nguyen, C. D., Curiel-Lewandrowski, C., & Kang, D. (2019). Low-cost, high-speed near infrared reflectance confocal microscope. Biomedical Optics Express, 10(7), 3497-3505.
JournalBIOMEDICAL OPTICS EXPRESS
RightsCopyright © 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractWe have developed a low-cost, near-infrared (NIR) reflectance confocal microscope (RCM) to overcome challenges in the imaging depth and speed found in our previously-reported smartphone confocal microscope. In the new NIR RCM device, we have used 840 nm superluminescent LED (sLED) to increase the tissue imaging depth and speed. A new confocal detection optics has been developed to maintain high lateral resolution even when a relatively large slit width was used. The material cost of the NIR RCM device was still low, similar to$5,200. The lateral resolution was 1.1 mu m and 1.3 mu m along the vertical and horizontal directions, respectively. Axial resolution was measured as 11.2 mu m. In vivo confocal images of human forearm skin obtained at the imaging speed of 203 frames/sec clearly visualized characteristic epidermal and dermal cellular features of the human skin. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
NoteOpen access journal
VersionFinal published version
SponsorsNational Institutes of Health/Fogarty International Center [R21TW010221]