A Novel, Highly Related Jumbo Family of Bacteriophages That Were Isolated Against Erwinia
Author
Sharma, RuchiraPielstick, Brittany A.
Bell, Kimberly A.
Nieman, Tanner B.
Stubbs, Olivia A.
Yeates, Edward L.
Baltrus, David A.
Grose, Julianne H.
Affiliation
Univ Arizona, Sch Plant SciIssue Date
2019-07-23
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Frontiers Media SACitation
Sharma R, Pielstick BA, Bell KA, Nieman TB, Stubbs OA, Yeates EL, Baltrus DA and Grose JH (2019) A Novel, Highly Related Jumbo Family of Bacteriophages That Were Isolated Against Erwinia. Front. Microbiol. 10:1533. doi: 10.3389/fmicb.2019.01533Journal
FRONTIERS IN MICROBIOLOGYRights
Copyright © 2019 Sharma, Pielstick, Bell, Nieman, Stubbs, Yeates, Baltrus and Grose. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Erwinia amylovora is a plant pathogen from the Erwiniaceae family and a causative agent of the devastating agricultural disease fire blight. Here we characterize eight lytic bacteriophages of E. amylovora that we isolated from the Wasatch front (Utah, United States) that are highly similar to vB_EamM_Ea35-70 which was isolated in Ontario, Canada. With the genome size ranging from 271 to 275 kb, this is a novel jumbo family of bacteriophages. These jumbo bacteriophages were further characterized through genomic and proteomic comparison, mass spectrometry, host range and burst size. Their proteomes are highly unstudied, with over 200 putative proteins with no known homologs. The production of 27 of these putative proteins was confirmed by mass spectrometry analysis. These bacteriophages appear to be most similar to bacteriophages that infect Pseudomonas and Ralstonia rather than Enterobacteriales bacteria by protein similarity, however, we were only able to detect infection of Erwinia and the closely related strains of Pantoea.Note
Open access journalISSN
1664-302XVersion
Final published versionSponsors
United States Dairy Association; University of Arizona; Brigham Young University; Department of Microbiology and Molecular Biology; College of Life Sciencesae974a485f413a2113503eed53cd6c53
10.3389/fmicb.2019.01533
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Except where otherwise noted, this item's license is described as Copyright © 2019 Sharma, Pielstick, Bell, Nieman, Stubbs, Yeates, Baltrus and Grose. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).

