A Novel, Highly Related Jumbo Family of Bacteriophages That Were Isolated Against Erwinia
Pielstick, Brittany A.
Bell, Kimberly A.
Nieman, Tanner B.
Stubbs, Olivia A.
Yeates, Edward L.
Baltrus, David A.
Grose, Julianne H.
AffiliationUniv Arizona, Sch Plant Sci
MetadataShow full item record
PublisherFrontiers Media SA
CitationSharma R, Pielstick BA, Bell KA, Nieman TB, Stubbs OA, Yeates EL, Baltrus DA and Grose JH (2019) A Novel, Highly Related Jumbo Family of Bacteriophages That Were Isolated Against Erwinia. Front. Microbiol. 10:1533. doi: 10.3389/fmicb.2019.01533
JournalFRONTIERS IN MICROBIOLOGY
RightsCopyright © 2019 Sharma, Pielstick, Bell, Nieman, Stubbs, Yeates, Baltrus and Grose. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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AbstractErwinia amylovora is a plant pathogen from the Erwiniaceae family and a causative agent of the devastating agricultural disease fire blight. Here we characterize eight lytic bacteriophages of E. amylovora that we isolated from the Wasatch front (Utah, United States) that are highly similar to vB_EamM_Ea35-70 which was isolated in Ontario, Canada. With the genome size ranging from 271 to 275 kb, this is a novel jumbo family of bacteriophages. These jumbo bacteriophages were further characterized through genomic and proteomic comparison, mass spectrometry, host range and burst size. Their proteomes are highly unstudied, with over 200 putative proteins with no known homologs. The production of 27 of these putative proteins was confirmed by mass spectrometry analysis. These bacteriophages appear to be most similar to bacteriophages that infect Pseudomonas and Ralstonia rather than Enterobacteriales bacteria by protein similarity, however, we were only able to detect infection of Erwinia and the closely related strains of Pantoea.
NoteOpen access journal
VersionFinal published version
SponsorsUnited States Dairy Association; University of Arizona; Brigham Young University; Department of Microbiology and Molecular Biology; College of Life Sciences