• Login
    View Item 
    •   Home
    • UA Faculty Research
    • UA Faculty Publications
    • View Item
    •   Home
    • UA Faculty Research
    • UA Faculty Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UA Campus RepositoryCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournal

    My Account

    LoginRegister

    About

    AboutUA Faculty PublicationsUA DissertationsUA Master's ThesesUA Honors ThesesUA PressUA YearbooksUA CatalogsUA Libraries

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    The effect of BPIFA1/SPLUNC1 genetic variation on its expression and function in asthmatic airway epithelium

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    127237.1-20190416152213-covere ...
    Size:
    764.8Kb
    Format:
    PDF
    Description:
    Final Published Version
    Download
    Author
    Schaefer, Niccolette
    Li, Xingnan
    Seibold, Max A
    Jarjour, Nizar N
    Denlinger, Loren C
    Castro, Mario
    Coverstone, Andrea M
    Teague, W Gerald
    Boomer, Jonathan
    Bleecker, Eugene R
    Meyers, Deborah A
    Moore, Wendy C
    Hawkins, Gregory A
    Fahy, John
    Phillips, Brenda R
    Mauger, David T
    Dakhama, Azzeddine
    Gellatly, Shaan
    Pavelka, Nicole
    Berman, Reena
    Di, Y Peter
    Wenzel, Sally E
    Chu, Hong Wei
    Show allShow less
    Affiliation
    Univ Arizona
    Issue Date
    2019-04-18
    
    Metadata
    Show full item record
    Publisher
    AMER SOC CLINICAL INVESTIGATION INC
    Citation
    Schaefer, N., Li, X., Seibold, M. A., Jarjour, N. N., Denlinger, L. C., Castro, M., ... & Meyers, D. A. (2019). The effect of BPIFA1/SPLUNC1 genetic variation on its expression and function in asthmatic airway epithelium. JCI insight, 4(8).
    Journal
    JCI INSIGHT
    Rights
    Copyright © 2019 American Society for Clinical Investigation.
    Collection Information
    This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
    Abstract
    Bacterial permeability family member A1 (BPIFA1), also known as short palate, lung, and nasal epithelium clone 1 (SPLUNC1), is a protein involved in the antiinflammatory response. The goal of this study was to determine whether BPIFA1 expression in asthmatic airways is regulated by genetic variations, altering epithelial responses to type 2 cytokines (e.g., IL-13). Nasal epithelial cells from patients with mild to severe asthma were collected from the National Heart, Lung. and Blood Institute Severe Asthma Research Program centers, genotyped for rs750064, and measured for BPIFA1. To determine the function of rs750064, cells were cultured at air-liquid interface and treated with 11-13 with or without recombinant human BPIFA1 (rhBPIFA1). Noncultured nasal cells with the rs750064 CC genotype had significantly less BPIFA1 mRNA expression than the CT and TT genotypes. Cultured CC versus CT and TT cells without stimulation maintained less BPIFA1 expression. With IL-13 treatment, CC genotype cells secreted more eotaxin-3 than CT and TT genotype cells. Also, rhBPIFA1 reduced IL-13-mediated eotaxin-3. BPIFA1 mRNA levels negatively correlated with serum IgE and fractional exhaled nitric oxide. Baseline FEV1% levels were lower in the asthma patients with the CC genotype (n = 1,016). Our data suggest that less BPIFA1 in asthma patients with the CC allele may predispose them to greater eosinophilic inflammation, which could be attenuated by rhBPIFA1 protein therapy.
    EISSN
    2379-3708
    PubMed ID
    30996135
    DOI
    10.1172/jci.insight.127237
    Version
    Final published version
    Sponsors
    NIH/NHLBI [R01HL125128, U10HL109257, UL1TR00448, U10HL109168]
    ae974a485f413a2113503eed53cd6c53
    10.1172/jci.insight.127237
    Scopus Count
    Collections
    UA Faculty Publications

    entitlement

    Related articles

    • Interleukin-13 Inhibits Lipopolysaccharide-Induced BPIFA1 Expression in Nasal Epithelial Cells.
    • Authors: Tsou YA, Lin CD, Chen HC, Hsu HY, Wu LT, Chiang-Ni C, Chen CJ, Wu TF, Kao MC, Chen YA, Peng MT, Tsai MH, Chen CM, Lai CH
    • Issue date: 2015
    • SPLUNC1 deficiency enhances airway eosinophilic inflammation in mice.
    • Authors: Thaikoottathil JV, Martin RJ, Di PY, Minor M, Case S, Zhang B, Zhang G, Huang H, Chu HW
    • Issue date: 2012 Aug
    • Expression of SPLUNC1 protein in nasal polyp epithelial cells in air-liquid interface culture treated with IL-13.
    • Authors: Yeh TH, Lee SY, Hsu WC
    • Issue date: 2010 Jan-Feb
    • Identification of BPIFA1/SPLUNC1 as an epithelium-derived smooth muscle relaxing factor.
    • Authors: Wu T, Huang J, Moore PJ, Little MS, Walton WG, Fellner RC, Alexis NE, Peter Di Y, Redinbo MR, Tilley SL, Tarran R
    • Issue date: 2017 Feb 6
    • Polymorphisms associated with expression of BPIFA1/BPIFB1 and lung disease severity in cystic fibrosis.
    • Authors: Saferali A, Obeidat M, Bérubé JC, Lamontagne M, Bossé Y, Laviolette M, Hao K, Nickle DC, Timens W, Sin DD, Postma DS, Strug LJ, Gallins PJ, Paré PD, Bingle CD, Sandford AJ
    • Issue date: 2015 Nov
    The University of Arizona Libraries | 1510 E. University Blvd. | Tucson, AZ 85721-0055
    Tel 520-621-6442 | repository@u.library.arizona.edu
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.