Serum Amyloid P Component Binds Fungal Surface Amyloid and Decreases Human Macrophage Phagocytosis and Secretion of Inflammatory Cytokines
AffiliationUniv Arizona, Dept Immunobiol
Univ Arizona, Dept Med
MetadataShow full item record
PublisherAMER SOC MICROBIOLOGY
CitationBehrens, N. E., Lipke, P. N., Pilling, D., Gomer, R. H., & Klotz, S. A. (2019). Serum Amyloid P Component Binds Fungal Surface Amyloid and Decreases Human Macrophage Phagocytosis and Secretion of Inflammatory Cytokines. mBio, 10(2), e00218-19.
RightsCopyright © 2019 Behrens et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractIn patients with invasive fungal diseases, there is often little cellular inflammatory response. We tested the idea that binding of the human constitutive plasma protein serum amyloid P component (SAP) (also called PTX2) to Candida albicans dampens the innate immune response to this fungus. Many pathogenic fungi have cell surface amyloid-like structures important for adhesion and biofilm formation. Human SAP bound to fungi that expressed functional cell surface amyloid, but SAP had minimal binding to fungi with reduced expression of cell surface amyloid. In the absence of SAP, phagocytosis of fungi by human macrophages was potentiated by expression of amyloid on the fungi. SAP binding to fungi inhibited their phagocytosis by macrophages. Macrophages pretreated with SAP displayed reduced fungal phagocytosis, reduced secretion of inflammatory cytokines (IFN-γ, IL-6, and TNF-α), and increased secretion of the anti-inflammatory cytokine IL-10. SAP bound to fungi or added to the medium upregulated the expression of the anti-inflammatory receptor CD206 on macrophages. These findings suggest that SAP bound to amyloid-like structures on fungal cells dampens the host cellular immune response in fungal diseases such as invasive candidiasis.IMPORTANCE Macrophages are a key part of our innate immune system and are responsible for recognizing invading microbes, ingesting them, and sending appropriate signals to other immune cells. We have found that human macrophages can recognize invading yeast pathogens that have a specific molecular pattern of proteins on their surfaces: these proteins have structures similar to the structures of amyloid aggregates in neurodegenerative diseases like Alzheimer's disease. However, this surface pattern also causes the fungi to bind a serum protein called serum amyloid P component (SAP). In turn, the SAP-coated yeasts are poorly recognized and seldom ingested by the macrophages, and the macrophages have a more tolerant and less inflammatory response in the presence of SAP. Therefore, we find that surface structures on the yeast can alter how the macrophages react to invading microbes.
NoteOpen access journal
VersionFinal published version
- New features of invasive candidiasis in humans: amyloid formation by fungi and deposition of serum amyloid P component by the host.
- Authors: Gilchrist KB, Garcia MC, Sobonya R, Lipke PN, Klotz SA
- Issue date: 2012 Nov
- Serum amyloid P component and C-reactive protein mediate phagocytosis through murine Fc gamma Rs.
- Authors: Mold C, Gresham HD, Du Clos TW
- Issue date: 2001 Jan 15
- Serum amyloid P component binds to late apoptotic cells and mediates their uptake by monocyte-derived macrophages.
- Authors: Bijl M, Horst G, Bijzet J, Bootsma H, Limburg PC, Kallenberg CG
- Issue date: 2003 Jan
- Structural recognition and functional activation of FcgammaR by innate pentraxins.
- Authors: Lu J, Marnell LL, Marjon KD, Mold C, Du Clos TW, Sun PD
- Issue date: 2008 Dec 18
- The Role of AIRE in the Immunity Against <i>Candida Albicans</i> in a Model of Human Macrophages.
- Authors: de Albuquerque JAT, Banerjee PP, Castoldi A, Ma R, Zurro NB, Ynoue LH, Arslanian C, Barbosa-Carvalho MUW, Correia-Deur JEM, Weiler FG, Dias-da-Silva MR, Lazaretti-Castro M, Pedroza LA, Câmara NOS, Mace E, Orange JS, Condino-Neto A
- Issue date: 2018