Use of rhodizonic acid for rapid detection of root border cell trapping of lead and reversal of trapping with DNase
AffiliationUniv Arizona, Dept Soil Water & Environm Sci
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CitationHuskey, D. A., G. Curlango-Rivera, and M. C. Hawes. 2019. Use of rhodizonic acid for rapid detection of root border cell trapping of lead and reversal of trapping with DNase. Applications in Plant Sciences 7(4): e1240.
JournalAPPLICATIONS IN PLANT SCIENCES
RightsCopyright © 2019 Huskey et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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AbstractPremise of the StudyLead (Pb) is a contaminant whose removal from soil remains a challenge. In a previous study, border cells released from root tips were found to trap Pb, alter its chemistry, and prevent root uptake. Rhodizonic acid (RA) is a forensic tool used to reveal gunshot residue, and also to detect Pb within plant tissues. Here we report preliminary observations to assess the potential application of RA in exploring the dynamics of Pb accumulation at the root tip surface. Methods and ResultsCorn root tips were immersed in Pb solution, stained with RA, and observed microscopically. Pb trapping by border cells was evident within minutes. The role of extracellular DNA was revealed when addition of nucleases resulted in dispersal of RA-stained Pb particles. ConclusionsRA is an efficient tool to monitor Pb-root interactions. Trapping by border cells may control Pb levels and chemistry at the root tip surface. Understanding how plants influence Pb distribution in soil may facilitate its remediation.
NoteOpen Access Journal
VersionFinal published version
SponsorsU.S. National Science Foundation [PBI-1457092]