• Login
    View Item 
    •   Home
    • UA Faculty Research
    • UA Faculty Publications
    • View Item
    •   Home
    • UA Faculty Research
    • UA Faculty Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UA Campus RepositoryCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournal

    My Account

    LoginRegister

    About

    AboutUA Faculty PublicationsUA DissertationsUA Master's ThesesUA Honors ThesesUA PressUA YearbooksUA CatalogsUA Libraries

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Transposon insertion causes cadherin mis-splicing and confers resistance to Bt cotton in pink bollworm from China

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    s41598-019-43889-x.pdf
    Size:
    1.901Mb
    Format:
    PDF
    Description:
    Final Published Version
    Download
    Author
    Wang, Ling
    Wang, Jintao
    Ma, Yuemin
    Wan, Peng
    Liu, Kaiyu
    Cong, Shengbo
    Xiao, Yutao
    Xu, Dong
    Wu, Kongming
    Fabrick, Jeffrey A
    Li, Xianchun
    Tabashnik, Bruce E
    Show allShow less
    Affiliation
    Univ Arizona, Dept Entomol
    Issue Date
    2019-05-16
    
    Metadata
    Show full item record
    Publisher
    NATURE PUBLISHING GROUP
    Citation
    Wang, L., Wang, J., Ma, Y., Wan, P., Liu, K., Cong, S., ... & Li, X. (2019). Transposon insertion causes cadherin mis-splicing and confers resistance to Bt cotton in pink bollworm from China. Scientific reports, 9(1), 7479.
    Journal
    SCIENTIFIC REPORTS
    Rights
    © The Author(s) 2019. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
    Collection Information
    This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
    Abstract
    Transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt) are cultivated extensively, but rapid evolution of resistance by pests reduces their efficacy. We report a 3,370-bp insertion in a cadherin gene associated with resistance to Bt toxin Cry1Ac in the pink bollworm (Pectinophora gossypiella), a devastating global cotton pest. We found the allele (r15) harboring this insertion in a field population from China. The insertion is a miniature inverted repeat transposable element (MITE) that contains two additional transposons and produces two mis-spliced transcript variants (r15A and r15B). A strain homozygous for r15 had 290-fold resistance to Cry1Ac, little or no cross-resistance to Cry2Ab, and completed its life cycle on Bt cotton producing Cry1Ac. Inheritance of resistance was recessive and tightly linked with r15. For transformed insect cells, susceptibility to Cry1Ac was greater for cells producing the wild-type cadherin than for cells producing the r15 mutant proteins. Recombinant cadherin protein occurred on the cell surface in cells transformed with the wildtype or r15A sequences, but not in cells transformed with the r15B sequence. The similar resistance of pink bollworm to Cry1Ac in laboratory-and field-selected insects from China, India and the U.S. provides a basis for developing international resistance management practices.
    Note
    Open access journal
    ISSN
    2045-2322
    PubMed ID
    31097777
    DOI
    10.1038/s41598-019-43889-x
    Version
    Final published version
    Sponsors
    China's Key Project for Breeding Genetically Modified Organisms [2016ZX08012-004]; Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2018-67013-27821]
    ae974a485f413a2113503eed53cd6c53
    10.1038/s41598-019-43889-x
    Scopus Count
    Collections
    UA Faculty Publications

    entitlement

    Related articles

    • Pink Bollworm Resistance to Bt Toxin Cry1Ac Associated with an Insertion in Cadherin Exon 20.
    • Authors: Wang L, Ma Y, Guo X, Wan P, Liu K, Cong S, Wang J, Xu D, Xiao Y, Li X, Tabashnik BE, Wu K
    • Issue date: 2019 Mar 28
    • Resistance to Bacillus thuringiensis linked with a cadherin transmembrane mutation affecting cellular trafficking in pink bollworm from China.
    • Authors: Wang L, Ma Y, Wan P, Liu K, Xiao Y, Wang J, Cong S, Xu D, Wu K, Fabrick JA, Li X, Tabashnik BE
    • Issue date: 2018 Mar
    • Alternative splicing and highly variable cadherin transcripts associated with field-evolved resistance of pink bollworm to bt cotton in India.
    • Authors: Fabrick JA, Ponnuraj J, Singh A, Tanwar RK, Unnithan GC, Yelich AJ, Li X, Carrière Y, Tabashnik BE
    • Issue date: 2014
    • Sustained susceptibility of pink bollworm to Bt cotton in the United States.
    • Authors: Tabashnik BE, Morin S, Unnithan GC, Yelich AJ, Ellers-Kirk C, Harpold VS, Sisterson MS, Ellsworth PC, Dennehy TJ, Antilla L, Liesner L, Whitlow M, Staten RT, Fabrick JA, Li X, Carrière Y
    • Issue date: 2012 Jul-Sep
    • Asymmetrical cross-resistance between Bacillus thuringiensis toxins Cry1Ac and Cry2Ab in pink bollworm.
    • Authors: Tabashnik BE, Unnithan GC, Masson L, Crowder DW, Li X, Carrière Y
    • Issue date: 2009 Jul 21
    The University of Arizona Libraries | 1510 E. University Blvd. | Tucson, AZ 85721-0055
    Tel 520-621-6442 | repository@u.library.arizona.edu
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.