Transposon insertion causes cadherin mis-splicing and confers resistance to Bt cotton in pink bollworm from China
Fabrick, Jeffrey A
Tabashnik, Bruce E
AffiliationUniv Arizona, Dept Entomol
MetadataShow full item record
PublisherNATURE PUBLISHING GROUP
CitationWang, L., Wang, J., Ma, Y., Wan, P., Liu, K., Cong, S., ... & Li, X. (2019). Transposon insertion causes cadherin mis-splicing and confers resistance to Bt cotton in pink bollworm from China. Scientific reports, 9(1), 7479.
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AbstractTransgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt) are cultivated extensively, but rapid evolution of resistance by pests reduces their efficacy. We report a 3,370-bp insertion in a cadherin gene associated with resistance to Bt toxin Cry1Ac in the pink bollworm (Pectinophora gossypiella), a devastating global cotton pest. We found the allele (r15) harboring this insertion in a field population from China. The insertion is a miniature inverted repeat transposable element (MITE) that contains two additional transposons and produces two mis-spliced transcript variants (r15A and r15B). A strain homozygous for r15 had 290-fold resistance to Cry1Ac, little or no cross-resistance to Cry2Ab, and completed its life cycle on Bt cotton producing Cry1Ac. Inheritance of resistance was recessive and tightly linked with r15. For transformed insect cells, susceptibility to Cry1Ac was greater for cells producing the wild-type cadherin than for cells producing the r15 mutant proteins. Recombinant cadherin protein occurred on the cell surface in cells transformed with the wildtype or r15A sequences, but not in cells transformed with the r15B sequence. The similar resistance of pink bollworm to Cry1Ac in laboratory-and field-selected insects from China, India and the U.S. provides a basis for developing international resistance management practices.
NoteOpen access journal
VersionFinal published version
SponsorsChina's Key Project for Breeding Genetically Modified Organisms [2016ZX08012-004]; Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2018-67013-27821]
- Pink Bollworm Resistance to Bt Toxin Cry1Ac Associated with an Insertion in Cadherin Exon 20.
- Authors: Wang L, Ma Y, Guo X, Wan P, Liu K, Cong S, Wang J, Xu D, Xiao Y, Li X, Tabashnik BE, Wu K
- Issue date: 2019 Mar 28
- Resistance to Bacillus thuringiensis linked with a cadherin transmembrane mutation affecting cellular trafficking in pink bollworm from China.
- Authors: Wang L, Ma Y, Wan P, Liu K, Xiao Y, Wang J, Cong S, Xu D, Wu K, Fabrick JA, Li X, Tabashnik BE
- Issue date: 2018 Mar
- Alternative splicing and highly variable cadherin transcripts associated with field-evolved resistance of pink bollworm to bt cotton in India.
- Authors: Fabrick JA, Ponnuraj J, Singh A, Tanwar RK, Unnithan GC, Yelich AJ, Li X, Carrière Y, Tabashnik BE
- Issue date: 2014
- Asymmetrical cross-resistance between Bacillus thuringiensis toxins Cry1Ac and Cry2Ab in pink bollworm.
- Authors: Tabashnik BE, Unnithan GC, Masson L, Crowder DW, Li X, Carrière Y
- Issue date: 2009 Jul 21
- Similar genetic basis of resistance to Bt toxin Cry1Ac in Boll-selected and diet-selected strains of pink bollworm.
- Authors: Fabrick JA, Tabashnik BE
- Issue date: 2012