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dc.contributor.authorWang, Ling
dc.contributor.authorWang, Jintao
dc.contributor.authorMa, Yuemin
dc.contributor.authorWan, Peng
dc.contributor.authorLiu, Kaiyu
dc.contributor.authorCong, Shengbo
dc.contributor.authorXiao, Yutao
dc.contributor.authorXu, Dong
dc.contributor.authorWu, Kongming
dc.contributor.authorFabrick, Jeffrey A
dc.contributor.authorLi, Xianchun
dc.contributor.authorTabashnik, Bruce E
dc.date.accessioned2019-08-30T23:04:41Z
dc.date.available2019-08-30T23:04:41Z
dc.date.issued2019-05-16
dc.identifier.citationWang, L., Wang, J., Ma, Y., Wan, P., Liu, K., Cong, S., ... & Li, X. (2019). Transposon insertion causes cadherin mis-splicing and confers resistance to Bt cotton in pink bollworm from China. Scientific reports, 9(1), 7479.en_US
dc.identifier.issn2045-2322
dc.identifier.pmid31097777
dc.identifier.doi10.1038/s41598-019-43889-x
dc.identifier.urihttp://hdl.handle.net/10150/634039
dc.description.abstractTransgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt) are cultivated extensively, but rapid evolution of resistance by pests reduces their efficacy. We report a 3,370-bp insertion in a cadherin gene associated with resistance to Bt toxin Cry1Ac in the pink bollworm (Pectinophora gossypiella), a devastating global cotton pest. We found the allele (r15) harboring this insertion in a field population from China. The insertion is a miniature inverted repeat transposable element (MITE) that contains two additional transposons and produces two mis-spliced transcript variants (r15A and r15B). A strain homozygous for r15 had 290-fold resistance to Cry1Ac, little or no cross-resistance to Cry2Ab, and completed its life cycle on Bt cotton producing Cry1Ac. Inheritance of resistance was recessive and tightly linked with r15. For transformed insect cells, susceptibility to Cry1Ac was greater for cells producing the wild-type cadherin than for cells producing the r15 mutant proteins. Recombinant cadherin protein occurred on the cell surface in cells transformed with the wildtype or r15A sequences, but not in cells transformed with the r15B sequence. The similar resistance of pink bollworm to Cry1Ac in laboratory-and field-selected insects from China, India and the U.S. provides a basis for developing international resistance management practices.en_US
dc.description.sponsorshipChina's Key Project for Breeding Genetically Modified Organisms [2016ZX08012-004]; Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2018-67013-27821]en_US
dc.language.isoenen_US
dc.publisherNATURE PUBLISHING GROUPen_US
dc.rights© The Author(s) 2019. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en_US
dc.titleTransposon insertion causes cadherin mis-splicing and confers resistance to Bt cotton in pink bollworm from Chinaen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Dept Entomolen_US
dc.identifier.journalSCIENTIFIC REPORTSen_US
dc.description.noteOpen access journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleScientific reports
refterms.dateFOA2019-08-30T23:04:41Z


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