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dc.contributor.authorPyon, Wonn S
dc.contributor.authorGray, Daniel T
dc.contributor.authorBarnes, Carol A
dc.date.accessioned2019-09-05T01:28:46Z
dc.date.available2019-09-05T01:28:46Z
dc.date.issued2019-07-18
dc.identifier.citationPyon WS, Gray DT and Barnes CA (2019) An Alternative to Dye-Based Approaches to Remove Background Autofluorescence From Primate Brain Tissue. Front. Neuroanat. 13:73. doi: 10.3389/fnana.2019.00073en_US
dc.identifier.issn1662-5129
dc.identifier.pmid31379520
dc.identifier.doi10.3389/fnana.2019.00073
dc.identifier.urihttp://hdl.handle.net/10150/634079
dc.description.abstractBrain tissue contains autofluorescing elements that potentially impede accurate identification of neurons when visualized with fluorescent microscopy. Age-related accumulation of molecules with autofluorescent properties, such as lipofuscin, can possess spectral profiles that invade the typical emission range of fluorophores commonly utilized in fluorescent microscopy. The traditional method for accounting for this native fluorescence is to apply lipophilic dyes that are able to sequester these unwanted signals. While effective, such dyes can present a range of problems including the obstruction of fluorescent probe emissions. The present study utilizes aged primate midbrain tissue stained for tyrosine hydroxylase and calbindin to investigate an image processing approach for removing autofluorescence utilizing spectral imaging and linear unmixing. This technique is then compared against the traditional, dye-based autofluorescence sequestration method using Sudan Black B (SBB). Spectral imaging and linear unmixing yielded significantly higher cell numbers than SBB treatment. This finding suggests that computational approaches for removing autofluorescence in neural tissue are both viable and preferential to dye-based approaches for estimation of cell body numbers.en_US
dc.description.sponsorshipNIA [R01 AG050548, P51 RR000169, F31 AG055263]; McKnight Brain Research Foundationen_US
dc.language.isoenen_US
dc.publisherFRONTIERS MEDIA SAen_US
dc.rightsCopyright © 2019 Pyon, Gray and Barnes. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectSudan Black Ben_US
dc.subjectautofluorescenceen_US
dc.subjectconfocal imagingen_US
dc.subjectfluorescence microscopyen_US
dc.subjectimmunohistochemistryen_US
dc.subjectlinear unmixingen_US
dc.subjectspectral imagingen_US
dc.titleAn Alternative to Dye-Based Approaches to Remove Background Autofluorescence From Primate Brain Tissueen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Evelyn F McKnight Brain Insten_US
dc.contributor.departmentUniv Arizona, ARL Div Neural Syst Memory & Agingen_US
dc.contributor.departmentUniv Arizona, Dept Psychol Neurol & Neuroscien_US
dc.identifier.journalFRONTIERS IN NEUROANATOMYen_US
dc.description.noteOpen access journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleFrontiers in neuroanatomy
refterms.dateFOA2019-09-05T01:28:47Z


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Copyright © 2019 Pyon, Gray and Barnes. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
Except where otherwise noted, this item's license is described as Copyright © 2019 Pyon, Gray and Barnes. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).