Integrated TORC1 and PKA signaling control the temporal activation of glucose-induced gene expression in yeast
AffiliationUniv Arizona, Dept Mol & Cellular Biol
MetadataShow full item record
PublisherNATURE PUBLISHING GROUP
CitationKunkel, J., Luo, X., & Capaldi, A. P. (2019). Integrated TORC1 and PKA signaling control the temporal activation of glucose-induced gene expression in yeast. Nature communications, 10(1), 1-11.
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AbstractThe growth rate of a yeast cell is controlled by the target of rapamycin kinase complex I (TORC1) and cAMP-dependent protein kinase (PKA) pathways. To determine how TORC1 and PKA cooperate to regulate cell growth, we performed temporal analysis of gene expression in yeast switched from a non-fermentable substrate, to glucose, in the presence and absence of TORC1 and PKA inhibitors. Quantitative analysis of these data reveals that PKA drives the expression of key cell growth genes during transitions into, and out of, the rapid growth state in glucose, while TORC1 is important for the steady-state expression of the same genes. This circuit design may enable yeast to set an exact growth rate based on the abundance of internal metabolites such as amino acids, via TORC1, but also adapt rapidly to changes in external nutrients, such as glucose, via PKA.
NoteOpen access journal
VersionFinal published version
SponsorsU.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) [1R01GM097329, T32GM084905]; NIGMS NIH HHS [T32 GM084905, R01 GM097329]
- Antagonistic interactions between the cAMP-dependent protein kinase and Tor signaling pathways modulate cell growth in Saccharomyces cerevisiae.
- Authors: Ramachandran V, Herman PK
- Issue date: 2011 Feb
- Transcription of the mating-type-regulated lncRNA IRT1 is governed by TORC1 and PKA.
- Authors: Moretto F, van Werven FJ
- Issue date: 2017 May
- Nutrient Control of Yeast Gametogenesis Is Mediated by TORC1, PKA and Energy Availability.
- Authors: Weidberg H, Moretto F, Spedale G, Amon A, van Werven FJ
- Issue date: 2016 Jun
- Protein kinase A, TOR, and glucose transport control the response to nutrient repletion in Saccharomyces cerevisiae.
- Authors: Slattery MG, Liko D, Heideman W
- Issue date: 2008 Feb
- The rapamycin-sensitive phosphoproteome reveals that TOR controls protein kinase A toward some but not all substrates.
- Authors: Soulard A, Cremonesi A, Moes S, Schütz F, Jenö P, Hall MN
- Issue date: 2010 Oct 1