Intraperitoneal injections as an alternative method for micro-CT contrast enhanced detection of murine liver tumors
Affiliation
Univ Arizona, Canc CtrUniv Arizona, Canc Biol Grad Interdisciplinary Program
Univ Arizona, Cell & Mol Med
Issue Date
2019-05-03
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FUTURE SCI LTDCitation
Sweeney, N., Marchant, S., & Martinez, J. D. (2019). Intraperitoneal injections as an alternative method for micro-CT contrast enhanced detection of murine liver tumors. BioTechniques, 66(5), 214-217.Journal
BIOTECHNIQUESRights
Copyright © 2019 Jesse D Martinez. This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Micro-computed tomography (micro-CT) coupled with tissue, or vascular, specific contrast agent has emerged as a powerful tool for detecting and monitoring tumor growth in the liver of murine animals. Intravenous injections of contrast agents can be technically challenging and lead to errors that can considerably influence the outcome of a preclinical study, prompting an alternative method. Here we assessed the effectiveness of intraperitoneal injections of polyiodinated triglycerides emulsions (FenestraLC) in micro-CT imaging of young SCID (8 weeks) and old BALB/c (48 weeks) mice with xenograft or carcinogen-induced liver tumors, respectively, and determined an optimal acquisition time. Utilizing an intraperitoneal injection is a viable alternative administration route for using Fenestrain detection and quantification of murine liver tumor burden. METHOD SUMMARY We report that intraperitoneal injections of polyiodinated triglycerides emulsions are a suitable alternative to the intravenous administration route in detecting and quantifying murine liver tumor burden.Note
Open access journalISSN
0736-6205PubMed ID
31050302Version
Final published versionSponsors
National Institute of Health [CA184920]; University of Arizona Cancer Center - National Cancer Institute [P30CA023074]ae974a485f413a2113503eed53cd6c53
10.2144/btn-2018-0162
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Except where otherwise noted, this item's license is described as Copyright © 2019 Jesse D Martinez. This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.
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