PKCζ and JNK signaling regulate radiation-induced compensatory proliferation in parotid salivary glands
AffiliationUniv Arizona, Canc Biol Grad Interdisciplinary Program
Univ Arizona, Dept Nutr Sci
MetadataShow full item record
PublisherPUBLIC LIBRARY SCIENCE
CitationWong WY, Allie S, Limesand KH (2019) PKCζ and JNK signaling regulate radiation-induced compensatory proliferation in parotid salivary glands. PLOS ONE 14(7): e0219572. https://doi.org/10.1371/journal.pone.0219572
RightsCopyright © 2019 Wong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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AbstractRadiotherapy is a common treatment option for head and neck cancer patients; however, the surrounding healthy salivary glands are often incidentally irradiated during the process. As a result, patients often experience persistent xerostomia and hyposalivation, which deceases their quality of life. Clinically, there is currently no standard of care available to restore salivary function. Repair of epithelial wounds involves cellular proliferation and establishment of polarity in order to regenerate the tissue. This process is partially mediated by protein kinase C zeta (PKC zeta), an apical polarity regulator; however, its role following radiation damage is not completely understood. Using an in vivo radiation model, we show a significant decrease in active PKC zeta in irradiated murine parotid glands, which correlates with increased proliferation that is sustained through 30 days post-irradiation. Additionally, salivary glands in PKC zeta null mice show increased basal proliferation which radiation treatment did not further potentiate. Radiation damage also activates Jun N-terminal kinase (JNK), a proliferation-inducing mitogen-activated protein kinase normally inhibited by PKC zeta. In both a PKC zeta null mouse model and in primary salivary gland cell cultures treated with a PKC zeta inhibitor, there was increased JNK activity and production of downstream proliferative transcripts. Collectively, these findings provide a potential molecular link by which PKC zeta suppression following radiation damage promotes JNK activation and radiation-induced compensatory proliferation in the salivary gland.
NoteOpen access journal
VersionFinal published version
SponsorsNIH [R01 DE023534]; Cancer Biology Training Grant [T32CA00921338]
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