Host-free biofilm culture of “Candidatus Liberibacter asiaticus,” the bacterium associated with Huanglongbing
AuthorHa, Phuc T.
Brown, Judith K.
Gang, David R.
AffiliationUniv Arizona, Sch Plant Sci
MetadataShow full item record
CitationHa, P. T., He, R., Killiny, N., Brown, J. K., Omsland, A., Gang, D. R., & Beyenal, H. (2019). Host-free biofilm culture of “Candidatus Liberibacter asiaticus,” the bacterium associated with Huanglongbing. Biofilm, 100005.
Rights© 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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AbstractInability to culture the phloem-restricted alpha-proteobacterium “Candidatus Liberibacter asiaticus” (“Ca. L. asiaticus”) or the closely related species (“Candidatus Liberibacter americanus” and “Candidatus Liberibacter africanus”) that are associated with Huanglongbing (HLB) hampers the development of effective long-term control strategies for this devastating disease. Here we report successful establishment and long-term maintenance of host-free “Ca. L. asiaticus” cultures, with the bacterium growing within cultured biofilms derived from infected citrus tissue. The biofilms were grown in a newly designed growth medium under specific conditions. The initial biofilm-based culture has been successfully maintained for over two years and has undergone over a dozen subcultures. Multiple independent cultures have been established and maintained in a biofilm reactor system, opening the door to the development of pure culture of “Ca. L. asiaticus” and the use of genetics-based methods to understand and mitigate the spread of HLB.
NoteOpen access journal
VersionFinal published version
SponsorsThis work was supported by the Citrus Disease Research and Extension Program of the Specialty Crop Research Initiative (grant no. 2016-70016-24824) of the USDA National Institute of Food and Agriculture.