Host-free biofilm culture of “Candidatus Liberibacter asiaticus,” the bacterium associated with Huanglongbing
Author
Ha, Phuc T.He, Ruifeng
Killiny, Nabil
Brown, Judith K.
Omsland, Anders
Gang, David R.
Beyenal, Haluk
Affiliation
Univ Arizona, Sch Plant SciIssue Date
2019-12
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Show full item recordPublisher
Elsevier BVCitation
Ha, P. T., He, R., Killiny, N., Brown, J. K., Omsland, A., Gang, D. R., & Beyenal, H. (2019). Host-free biofilm culture of “Candidatus Liberibacter asiaticus,” the bacterium associated with Huanglongbing. Biofilm, 100005.Journal
BIOFILMRights
© 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Inability to culture the phloem-restricted alpha-proteobacterium “Candidatus Liberibacter asiaticus” (“Ca. L. asiaticus”) or the closely related species (“Candidatus Liberibacter americanus” and “Candidatus Liberibacter africanus”) that are associated with Huanglongbing (HLB) hampers the development of effective long-term control strategies for this devastating disease. Here we report successful establishment and long-term maintenance of host-free “Ca. L. asiaticus” cultures, with the bacterium growing within cultured biofilms derived from infected citrus tissue. The biofilms were grown in a newly designed growth medium under specific conditions. The initial biofilm-based culture has been successfully maintained for over two years and has undergone over a dozen subcultures. Multiple independent cultures have been established and maintained in a biofilm reactor system, opening the door to the development of pure culture of “Ca. L. asiaticus” and the use of genetics-based methods to understand and mitigate the spread of HLB.Note
Open access journalISSN
2590-2075Version
Final published versionSponsors
This work was supported by the Citrus Disease Research and Extension Program of the Specialty Crop Research Initiative (grant no. 2016-70016-24824) of the USDA National Institute of Food and Agriculture.ae974a485f413a2113503eed53cd6c53
10.1016/j.bioflm.2019.100005
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Except where otherwise noted, this item's license is described as © 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).