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    Host-free biofilm culture of “Candidatus Liberibacter asiaticus,” the bacterium associated with Huanglongbing

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    Author
    Ha, Phuc T.
    He, Ruifeng
    Killiny, Nabil
    Brown, Judith K.
    Omsland, Anders
    Gang, David R.
    Beyenal, Haluk
    Affiliation
    Univ Arizona, Sch Plant Sci
    Issue Date
    2019-12
    Keywords
    Biofilm
    Host-free culture
    Isolation
    Candidatus Liberibacter asiaticus
    Huanglongbing
    
    Metadata
    Show full item record
    Publisher
    Elsevier BV
    Citation
    Ha, P. T., He, R., Killiny, N., Brown, J. K., Omsland, A., Gang, D. R., & Beyenal, H. (2019). Host-free biofilm culture of “Candidatus Liberibacter asiaticus,” the bacterium associated with Huanglongbing. Biofilm, 100005.
    Journal
    BIOFILM
    Rights
    © 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
    Collection Information
    This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
    Abstract
    Inability to culture the phloem-restricted alpha-proteobacterium “Candidatus Liberibacter asiaticus” (“Ca. L. asiaticus”) or the closely related species (“Candidatus Liberibacter americanus” and “Candidatus Liberibacter africanus”) that are associated with Huanglongbing (HLB) hampers the development of effective long-term control strategies for this devastating disease. Here we report successful establishment and long-term maintenance of host-free “Ca. L. asiaticus” cultures, with the bacterium growing within cultured biofilms derived from infected citrus tissue. The biofilms were grown in a newly designed growth medium under specific conditions. The initial biofilm-based culture has been successfully maintained for over two years and has undergone over a dozen subcultures. Multiple independent cultures have been established and maintained in a biofilm reactor system, opening the door to the development of pure culture of “Ca. L. asiaticus” and the use of genetics-based methods to understand and mitigate the spread of HLB.
    Note
    Open access journal
    ISSN
    2590-2075
    DOI
    10.1016/j.bioflm.2019.100005
    Version
    Final published version
    Sponsors
    This work was supported by the Citrus Disease Research and Extension Program of the Specialty Crop Research Initiative (grant no. 2016-70016-24824) of the USDA National Institute of Food and Agriculture.
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.bioflm.2019.100005
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