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dc.contributor.authorHa, Phuc T.
dc.contributor.authorHe, Ruifeng
dc.contributor.authorKilliny, Nabil
dc.contributor.authorBrown, Judith K.
dc.contributor.authorOmsland, Anders
dc.contributor.authorGang, David R.
dc.contributor.authorBeyenal, Haluk
dc.date.accessioned2019-09-25T22:43:25Z
dc.date.available2019-09-25T22:43:25Z
dc.date.issued2019-12
dc.identifier.citationHa, P. T., He, R., Killiny, N., Brown, J. K., Omsland, A., Gang, D. R., & Beyenal, H. (2019). Host-free biofilm culture of “Candidatus Liberibacter asiaticus,” the bacterium associated with Huanglongbing. Biofilm, 100005.en_US
dc.identifier.issn2590-2075
dc.identifier.doi10.1016/j.bioflm.2019.100005
dc.identifier.urihttp://hdl.handle.net/10150/634573
dc.description.abstractInability to culture the phloem-restricted alpha-proteobacterium “Candidatus Liberibacter asiaticus” (“Ca. L. asiaticus”) or the closely related species (“Candidatus Liberibacter americanus” and “Candidatus Liberibacter africanus”) that are associated with Huanglongbing (HLB) hampers the development of effective long-term control strategies for this devastating disease. Here we report successful establishment and long-term maintenance of host-free “Ca. L. asiaticus” cultures, with the bacterium growing within cultured biofilms derived from infected citrus tissue. The biofilms were grown in a newly designed growth medium under specific conditions. The initial biofilm-based culture has been successfully maintained for over two years and has undergone over a dozen subcultures. Multiple independent cultures have been established and maintained in a biofilm reactor system, opening the door to the development of pure culture of “Ca. L. asiaticus” and the use of genetics-based methods to understand and mitigate the spread of HLB.en_US
dc.description.sponsorshipThis work was supported by the Citrus Disease Research and Extension Program of the Specialty Crop Research Initiative (grant no. 2016-70016-24824) of the USDA National Institute of Food and Agriculture.en_US
dc.language.isoenen_US
dc.publisherElsevier BVen_US
dc.rights© 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).en_US
dc.subjectBiofilmen_US
dc.subjectHost-free cultureen_US
dc.subjectIsolationen_US
dc.subjectCandidatus Liberibacter asiaticusen_US
dc.subjectHuanglongbingen_US
dc.titleHost-free biofilm culture of “Candidatus Liberibacter asiaticus,” the bacterium associated with Huanglongbingen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Sch Plant Scien_US
dc.identifier.journalBIOFILMen_US
dc.description.noteOpen access journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.volume1
dc.source.beginpage100005
refterms.dateFOA2019-09-25T22:43:25Z


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