Characterization of an Uncinocarpus reesii-expressed recombinant tube precipitin antigen of Coccidioides posadasii for serodiagnosis
dc.contributor.author | Yu, Jieh-Juen | |
dc.contributor.author | Holbrook, Eric | |
dc.contributor.author | Liao, Yu-Rou | |
dc.contributor.author | Zarnowski, Robert | |
dc.contributor.author | Andes, David R | |
dc.contributor.author | Wheat, L Joseph | |
dc.contributor.author | Malo, Joshua | |
dc.contributor.author | Hung, Chiung-Yu | |
dc.date.accessioned | 2019-10-09T21:36:35Z | |
dc.date.available | 2019-10-09T21:36:35Z | |
dc.date.issued | 2019-08-14 | |
dc.identifier.citation | Yu J-J, Holbrook E, Liao Y-R, Zarnowski R, Andes DR, Wheat LJ, et al. (2019) Characterization of an Uncinocarpus reesii-expressed recombinant tube precipitin antigen of Coccidioides posadasii for serodiagnosis. PLoS ONE 14(8): e0221228. https://doi.org/10.1371/journal.pone.0221228 | en_US |
dc.identifier.issn | 1932-6203 | |
dc.identifier.pmid | 31412087 | |
dc.identifier.doi | 10.1371/journal.pone.0221228 | |
dc.identifier.uri | http://hdl.handle.net/10150/634729 | |
dc.description.abstract | Early and accurate diagnosis of coccidioidomycosis, also known as Valley fever, is critical for appropriate disease treatment and management. Current serodiagnosis is based on the detection of patient serum antibodies that react with tube precipitin (TP) and complement fixation (CF) antigens of Coccidioides. IgM is the first class of antibodies produced by hosts in response to coccidioidal insults. The highly glycosylated beta-glucosidase 2 (BGL2) is a major active component of the TP antigen that stimulates IgM antibody responses during early Coccidioides infection. The predominant IgM epitope on BGL2 is a unique 3-O-methylmannose moiety that is not produced by commonly used protein expression systems. We genetically engineered and expressed a recombinant BGL2 (rBGL2ur), derived from Coccidioides, in non-pathogenic Uncinocarpus reesii, a fungus phylogenetically related to the Coccidioides pathogen. The rBGL2ur protein was purified from the culture medium of transformed U. reesii by nickel affinity chromatography, and the presence of 3-O-methyl man nose was demonstrated by gas chromatography. Seroreactivity of the purified rBGL2ur protein was tested by enzyme-linked immunosorbent assays using sera from 90 patients with coccidioidomycosis and 134 control individuals. The sensitivity and specificity of the assay with rBGL2ur were 78.8% and 87.3%, respectively. These results were comparable to those obtained using a proprietary MiraVista Diagnostic (MVD) IgM (63.3% sensitivity; 96.3% specificity), but significantly better than the ID-TP assay using non-concentrated patient sera (33.3% sensitivity; 100% specificity). Expression of rBGL2ur in U. reesii retains its antigenicity for coccidioidomycosis serodiagnosis and greatly reduces biosafety concerns for antigen production, as Coccidioides spp. are biological safety level 3 agents. | en_US |
dc.description.sponsorship | National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R21A1114762, R01A1135005, R43 A1131868-01]; MiraVista Diagnostics (MVD) | en_US |
dc.language.iso | en | en_US |
dc.publisher | PUBLIC LIBRARY SCIENCE | en_US |
dc.rights | Copyright © 2019 Yu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en_US |
dc.title | Characterization of an Uncinocarpus reesii-expressed recombinant tube precipitin antigen of Coccidioides posadasii for serodiagnosis | en_US |
dc.type | Article | en_US |
dc.contributor.department | Univ Arizona, Coll Med | en_US |
dc.identifier.journal | PLOS ONE | en_US |
dc.description.note | Open access journal | en_US |
dc.description.collectioninformation | This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu. | en_US |
dc.eprint.version | Final published version | en_US |
dc.source.journaltitle | PloS one | |
refterms.dateFOA | 2019-10-09T21:36:36Z |