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dc.contributor.authorCole, Bryan K
dc.contributor.authorIlikj, Marko
dc.contributor.authorMcCloskey, Cindy B
dc.contributor.authorChavez-Bueno, Susana
dc.date.accessioned2019-10-09T21:49:09Z
dc.date.available2019-10-09T21:49:09Z
dc.date.issued2019-07-05
dc.identifier.citationCole BK, Ilikj M, McCloskey CB, Chavez-Bueno S (2019) Antibiotic resistance and molecular characterization of bacteremia Escherichia coli isolates from newborns in the United States. PLoS ONE 14(7): e0219352. https://doi.org/10.1371/journal.pone.0219352en_US
dc.identifier.issn1932-6203
dc.identifier.pmid31276562
dc.identifier.doi10.1371/journal.pone.0219352
dc.identifier.urihttp://hdl.handle.net/10150/634731
dc.description.abstractBackground Escherichia coli is a major cause of neonatal sepsis. Contemporary antibiotic resistance data and molecular characterization of neonatal E. coli bacteremia isolates in the US are limited. Methods E. coli blood isolates, antibiotic susceptibility data, and clinical characteristics were obtained from prospectively identified newborns from 2006 to 2016. The E. coli isolates were classified using an updated phylogrouping method and multi-locus sequence typing. The presence of several virulence traits was also determined. Results Forty-three newborns with E. coli bacteremia were identified. Mean gestational age was 32.3 (SD +/- 5.4) weeks. Median age was 7 days (interquartile range 0-10). Mortality (28%) occurred exclusively in preterm newborns. Resistance to ampicillin was 67%, to gentamicin was 14%, and to ceftriaxone was 2%; one isolate produced extended-spectrum beta lacta-mases. Phylogroup B2 predominated. Sequence type (ST) 95 and ST131 prevailed; ST1193 emerged recently. All isolates carried fimH, nlpI, and ompA, and 46% carried the K1 capsule. E. coli from newborns with bacteremia diagnosed at <72 hours old had more virulence genes compared to E. coli from newborns. 72 hours old. The hek/hra gene was more frequent in isolates from newborns who died than in isolates from survivors. Conclusion Antibiotic resistance in E. coli was prevalent in this large collection of bacteremia isolates from US newborns. Most strains belonged to distinctive extra-intestinal pathogenic E. coil phylogroups and STs. Further characterization of virulence genes in neonatal E. coli bacteremia strains is needed in larger numbers and in more geographically diverse areas.en_US
dc.description.sponsorshipOklahoma INBRE program [8P20GM103447]; NIH/NIGMSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS)en_US
dc.language.isoenen_US
dc.publisherPUBLIC LIBRARY SCIENCEen_US
dc.rightsCopyright © 2019 Cole et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.titleAntibiotic resistance and molecular characterization of bacteremia Escherichia coli isolates from newborns in the United Statesen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Dept Child Hlthen_US
dc.identifier.journalPLOS ONEen_US
dc.description.noteOpen access journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitlePloS one
refterms.dateFOA2019-10-09T21:49:10Z


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