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dc.contributor.authorAlmeida, Jamie L
dc.contributor.authorDakic, Aleksandra
dc.contributor.authorKindig, Karin
dc.contributor.authorKone, Maikan
dc.contributor.authorLetham, Deborah L D
dc.contributor.authorLangdon, Scott
dc.contributor.authorPeat, Ruth
dc.contributor.authorHolding-Pillai, Jayamalini
dc.contributor.authorHall, Erin M
dc.contributor.authorLadd, Mark
dc.contributor.authorShaffer, Megan D
dc.contributor.authorBerg, Heath
dc.contributor.authorLi, Jinliang
dc.contributor.authorWigger, Georges
dc.contributor.authorLund, Steve
dc.contributor.authorSteffen, Carolyn R
dc.contributor.authorFransway, Barbara B
dc.contributor.authorGeraghty, Bob
dc.contributor.authorNatoli, Manuela
dc.contributor.authorBauer, Beth
dc.contributor.authorGollin, Susanne M
dc.contributor.authorLewis, Dale W
dc.contributor.authorReid, Yvonne
dc.date.accessioned2019-10-09T22:07:10Z
dc.date.available2019-10-09T22:07:10Z
dc.date.issued2019-06-20
dc.identifier.citationAlmeida JL, Dakic A, Kindig K, Kone M,Letham DLD,Langdon S, et al. (2019) Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines. PLoS ONE 14(6): e0218412. https://doi.org/10.1371/journal.pone.0218412en_US
dc.identifier.issn1932-6203
dc.identifier.pmid31220119
dc.identifier.doi10.1371/journal.pone.0218412
dc.identifier.urihttp://hdl.handle.net/10150/634733
dc.description.abstractThe Consortium for Mouse Cell Line Authentication was formed to validate Short Tandem Repeat (STR) markers for intraspecies identification of mouse cell lines. The STR profiling method is a multiplex polymerase chain reaction (PCR) assay comprised of primers targeting 19 mouse STR markers and two human STR markers (for interspecies contamination screening). The goals of the Consortium were to perform an interlaboratory study to-(1) validate the mouse STR markers to uniquely identify mouse cell lines (intraspecies identification), (2) to provide a public database of mouse cell lines with the National Institute of Standards and Technology (NIST)-validated mouse STR profiles, and (3) to publish the results of the interlaboratory study. The interlaboratory study was an international effort that consisted of 12 participating laboratories representing institutions from academia, industry, biological resource centers, and government. The study was based on 50 of the most commonly used mouse cell lines obtained from the American Type Culture Collection (ATCC). Of the 50 mouse cell lines, 18 had unique STR profiles that were 100% concordant (match) among all Consortium laboratory members, and the remaining 32 cell lines had discordance that was resolved readily and led to improvement of the assay. The discordance was due to low signal and interpretation issues involving artifacts and genotyping errors. Although the total number of discordant STR profiles was relatively high in this study, the percent of labs agreeing on allele calls among the discordant samples was above 92%. The STR profiles, including electropherogram images, for NIST-validated mouse cell lines will be published on the NCBI BioSample Database (https://www.ncbi.nlm.nih.gov/biosample/). Overall, the interlaboratory study showed that the multiplex PCR method using 18 of the 19 mouse STR markers is capable of discriminating at the intraspecies level between mouse cell lines. Further studies are ongoing to refine the assay including (1) development of an allelic ladder for improving the accuracy of allele calling and (2) integration of stutter filters to identify true stutter.en_US
dc.language.isoenen_US
dc.publisherPUBLIC LIBRARY SCIENCEen_US
dc.rightsThis is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.en_US
dc.titleInterlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell linesen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Genet Core, Genet Core Laben_US
dc.identifier.journalPLOS ONEen_US
dc.description.noteOpen access journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitlePloS one
refterms.dateFOA2019-10-09T22:07:11Z


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