Non-canonical transcriptional regulation of heme oxygenase in Aedes aegypti
Pereira, Luiza O R
Talyuli, Octavio A C
Dunkov, Boris C
Oliveira, Pedro L
Paiva-Silva, Gabriela O
AffiliationUniv Arizona, Ctr Insect Sci
MetadataShow full item record
PublisherNATURE PUBLISHING GROUP
CitationBottino-Rojas, V., Pereira, L. O., Silva, G., Talyuli, O. A., Dunkov, B. C., Oliveira, P. L., & Paiva-Silva, G. O. (2019). Non-canonical transcriptional regulation of heme oxygenase in Aedes aegypti. Scientific reports, 9(1), 1-12.
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AbstractHeme oxygenase (HO) is a ubiquitous enzyme responsible for heme breakdown, which yields carbon monoxide (CO), biliverdin (BV) and ferrous ion. Here we show that the Aedes aegypti heme oxygenase gene (AeHO-AAEL008136) is expressed in different developmental stages and tissues. AeHO expression increases after a blood meal in the midgut, and its maximal transcription levels overlaps with the maximal rate of the further modified A. aegypti biglutaminyl-biliverdin (AeBV) pigment production. HO is a classical component of stress response in eukaryotic cells, being activated under oxidative stress or increased heme levels. Indeed, the final product of HO activity in the mosquito midgut, AeBV, exerts a protective antioxidant activity. AeHO, however, does not seem to be under a classical redox-sensitive transcriptional regulation, being unresponsive to heme itself, and even down regulated when insects face a pro-oxidant insult. In contrast, AeHO gene expression responds to nutrient sensing mechanisms, through the target of rapamycin (TOR) pathway. This unusual transcriptional control of AeHO, together with the antioxidant properties of AeBV, suggests that heme degradation by HO, in addition to its important role in protection of Aedes aegypti against heme exposure, also acts as a digestive feature, being an essential adaptation to blood feeding.
NoteOpen access journal
VersionFinal published version
SponsorsConselho Nacional de Desenvolvimento Cientifico e TecnologicoNational Council for Scientific and Technological Development (CNPq); Coordenacao de Aperfeicoamento de Pessoal de Nivel SuperiorCAPES; National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [GM58918]; Fundacao de Amparo a Pesquisa do Estado do Rio de JaneiroCarlos Chagas Filho Foundation for Research Support of the State of Rio de Janeiro (FAPERJ)
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- Authors: Pereira LO, Oliveira PL, Almeida IC, Paiva-Silva GO
- Issue date: 2007 Jun 12
- Heme oxygenase-1 inhibition of MAP kinases, calcineurin/NFAT signaling, and hypertrophy in cardiac myocytes.
- Authors: Tongers J, Fiedler B, König D, Kempf T, Klein G, Heineke J, Kraft T, Gambaryan S, Lohmann SM, Drexler H, Wollert KC
- Issue date: 2004 Aug 15
- Role of Heme Oxygenase as a Modulator of Heme-Mediated Pathways.
- Authors: Duvigneau JC, Esterbauer H, Kozlov AV
- Issue date: 2019 Oct 11
- Blood meal-derived heme decreases ROS levels in the midgut of Aedes aegypti and allows proliferation of intestinal microbiota.
- Authors: Oliveira JH, Gonçalves RL, Lara FA, Dias FA, Gandara AC, Menna-Barreto RF, Edwards MC, Laurindo FR, Silva-Neto MA, Sorgine MH, Oliveira PL
- Issue date: 2011 Mar
- The Janus face of the heme oxygenase/biliverdin reductase system in Alzheimer disease: it's time for reconciliation.
- Authors: Barone E, Di Domenico F, Mancuso C, Butterfield DA
- Issue date: 2014 Feb