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dc.contributor.authorHegedűsová, Eva
dc.contributor.authorKulkarni, Sneha
dc.contributor.authorBurgman, Brandon
dc.contributor.authorAlfonzo, Juan D
dc.contributor.authorParis, Zdeněk
dc.date.accessioned2019-11-07T03:14:13Z
dc.date.available2019-11-07T03:14:13Z
dc.date.issued2019-09-19
dc.identifier.citationEva Hegedűsová, Sneha Kulkarni, Brandon Burgman, Juan D Alfonzo, Zdeněk Paris, The general mRNA exporters Mex67 and Mtr2 play distinct roles in nuclear export of tRNAs in Trypanosoma brucei, Nucleic Acids Research, Volume 47, Issue 16, 19 September 2019, Pages 8620–8631, https://doi.org/10.1093/nar/gkz671en_US
dc.identifier.issn0305-1048
dc.identifier.pmid31392978
dc.identifier.doi10.1093/nar/gkz671
dc.identifier.urihttp://hdl.handle.net/10150/634992
dc.description.abstractTransfer RNAs (tRNAs) are central players in protein synthesis, which in Eukarya need to be delivered from the nucleus to the cytoplasm by specific transport receptors, most of which belong to the evolutionarily conserved beta-importin family. Based on the available literature, we identified two candidates, Xpo-t and Xpo-5 for tRNA export in Trypanosoma brucei. However, down-regulation of expression of these genes did not disrupt the export of tRNAs to the cytoplasm. In search of alternative pathways, we tested the mRNA export complex Mex67-Mtr2, for a role in tRNA nuclear export, as described previously in yeast. Down-regulation of either exporter affected the subcellular distribution of tRNAs. However, contrary to yeast, TbMex67 and TbMtr2 accumulated different subsets of tRNAs in the nucleus. While TbMtr2 perturbed the export of all the tRNAs tested, silencing of TbMex67, led to the nuclear accumulation of tRNAs that are typically modified with queuosine. In turn, inhibition of tRNA nuclear export also affected the levels of queuosine modification in tRNAs. Taken together, the results presented demonstrate the dynamic nature of tRNA trafficking in T. brucei and its potential impact not only on the availability of tRNAs for protein synthesis but also on their modification status.en_US
dc.description.sponsorshipUnited States Department of Health & Human Services, National Institutes of Health (NIH) - USA [GM084065-10, AI131248]; Czech Science Foundation, Grant Agency of the Czech Republic [15-21450Y]; ERDF/ESF project Centre for Research of Pathogenicity and Virulence of Parasites [CZ.02.1.01/0.0/0.0/16 019/0000759]; University of South Bohemia [036/2017/P]; University of Arizona [NIH MHIRT T37 MD001427]; Biology Centre CAS, Institute of Parasitologyen_US
dc.language.isoenen_US
dc.publisherOXFORD UNIV PRESSen_US
dc.rightsCopyright © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.comen_US
dc.titleThe general mRNA exporters Mex67 and Mtr2 play distinct roles in nuclear export of tRNAs in Trypanosoma bruceien_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizonaen_US
dc.identifier.journalNUCLEIC ACIDS RESEARCHen_US
dc.description.noteOpen access journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleNucleic acids research
refterms.dateFOA2019-11-07T03:14:14Z


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