Show simple item record

dc.contributor.authorHall, Branwen M
dc.contributor.authorRoberts, Sue A
dc.contributor.authorCordes, Matthew H J
dc.date.accessioned2019-11-07T19:14:58Z
dc.date.available2019-11-07T19:14:58Z
dc.date.issued2019-07-26
dc.identifier.citationBranwen M Hall, Sue A Roberts, Matthew H J Cordes, Extreme divergence between one-to-one orthologs: the structure of N15 Cro bound to operator DNA and its relationship to the λ Cro complex, Nucleic Acids Research, Volume 47, Issue 13, 26 July 2019, Pages 7118–7129, https://doi.org/10.1093/nar/gkz507en_US
dc.identifier.issn0305-1048
dc.identifier.pmid31180482
dc.identifier.doi10.1093/nar/gkz507
dc.identifier.urihttp://hdl.handle.net/10150/635028
dc.description.abstractThe gene cro promotes lytic growth of phages through binding of Cro protein dimers to regulatory DNA sites. Most Cro proteins are one-to-one orthologs, yet their sequence, structure and binding site sequences are quite divergent across lambdoid phages. We report the cocrystal structure of bacteriophage N15 Cro with a symmetric consensus site. We contrast this complex with an orthologous structure from phage λ, which has a dissimilar binding site sequence and a Cro protein that is highly divergent in sequence, dimerization interface and protein fold. The N15 Cro complex has less DNA bending and smaller DNA-induced changes in protein structure. N15 Cro makes fewer direct contacts and hydrogen bonds to bases, relying mostly on water-mediated and Van der Waals contacts to recognize the sequence. The recognition helices of N15 Cro and λ Cro make mostly nonhomologous and nonanalogous contacts. Interface alignment scores show that half-site binding geometries of N15 Cro and λ Cro are less similar to each other than to distantly related CI repressors. Despite this divergence, the Cro family shows several code-like protein–DNA sequence covariations. In some cases, orthologous genes can achieve a similar biological function using very different specific molecular interactions.en_US
dc.description.sponsorshipNational Science Foundation (NSF) [MCB0643790]; University of Arizonaen_US
dc.language.isoenen_US
dc.publisherOXFORD UNIV PRESSen_US
dc.rightsCopyright © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.comen_US
dc.titleExtreme divergence between one-to-one orthologs: the structure of N15 Cro bound to operator DNA and its relationship to the λ Cro complexen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Dept Chem & Biochemen_US
dc.identifier.journalNUCLEIC ACIDS RESEARCHen_US
dc.description.noteOpen access journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleNucleic acids research
refterms.dateFOA2019-11-07T19:14:59Z


Files in this item

Thumbnail
Name:
gkz507(1).pdf
Size:
4.653Mb
Format:
PDF
Description:
Final Published Version

This item appears in the following Collection(s)

Show simple item record