• Improved Collagen Extraction by Modified Longin Method

      Brown, T. A.; Nelson, D. E.; Vogel, J. S.; Southon, J. R. (American Journal of Science, 1988-01-01)
      A re-evaluation of the Longin collagen-extraction method shows that a lower reflux temperature reduces degradation of protein ("collagen") remnants. This allows additional purification through ultrafiltration to isolate the >30kDalton fraction of the reflux product.