Development and Characterization of An Injury-free Model of Functional Pain in Rats by Exposure to Red Light
Chew, Lindsey A
Bellampalli, Shreya S
Schmoll, Ryan W
Vanderah, Todd W
Ibrahim, Mohab M
AffiliationUniv Arizona, Dept Anesthesiol
Univ Arizona, Dept Pharmacol
Univ Arizona, Coll Med, Dept Grad Interdisciplinary Program Neurosci
KeywordsFunctional pain syndromes
red light-emitting diode
MetadataShow full item record
CitationKhanna, R., Patwardhan, A., Yang, X., Li, W., Cai, S., Ji, Y., ... & Gordon, J. (2019). Development and Characterization of An Injury-free Model of Functional Pain in Rats by Exposure to Red Light. The Journal of Pain.
JournalJOURNAL OF PAIN
Rights© 2019 by the American Pain Society
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractWe report the development and characterization of a novel, injury-free rat model in which nociceptive sensitization after red light is observed in multiple body areas reminiscent of widespread pain in functional pain syndromes. Rats were exposed to red light-emitting diodes (RLED) (LEDs, 660 nm) at an intensity of 50 Lux for 8 hours daily for 5 days resulting in time- and dose-dependent thermal hyperalgesia and mechanical allodynia in both male and female rats. Females showed an earlier onset of mechanical allodynia than males. The pronociceptive effects of RLED were mediated through the visual system. RLED-induced thermal hyperalgesia and mechanical allodynia were reversed with medications commonly used for widespread pain, including gabapentin, tricyclic antidepressants, serotonin/norepinephrine reuptake inhibitors, and nonsteroidal anti-inflammatory drugs. Acetaminophen failed to reverse the RLED induced hypersensitivity. The hyperalgesic effects of RLED were blocked when bicuculline, a gamma-aminobutyric acid-A receptor antagonist, was administered into the rostra! ventromedial medulla, suggesting a role for increased descending facilitation in the pain pathway. Key experiments were subjected to a replication study with randomization, investigator blinding, inclusion of all data, and high levels of statistical rigor. RLED-induced thermal hyperalgesia and mechanical allodynia without injury offers a novel injury-free rodent model useful for the study of functional pain syndromes with widespread pain. RLED exposure also emphasizes the different biological effects of different colors of light exposure. Perspective: This study demonstrates the effect of light exposure on nociceptive thresholds. These biological effects of red LED add evidence to the emerging understanding of the biological effects of light of different colors in animals and humans. Understanding the underlying biology of red light-induced widespread pain may offer insights into functional pain states. (C) 2019 by the American Pain Society
Note12 month embargo; published online: 2 May 2019
VersionFinal accepted manuscript
SponsorsNational Center for Complementary and Alternative Health [R01AT009716]; National Institute for Neurological Disorders and StrokeUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Neurological Disorders & Stroke (NINDS) [1R01N5098772]; NINDSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Neurological Disorders & Stroke (NINDS); Children's Tumor Foundation; University of Arizona
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