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dc.contributor.authorMikell, Iliyana
dc.contributor.authorCrawford, Lindsey B
dc.contributor.authorHancock, Meaghan H
dc.contributor.authorMitchell, Jennifer
dc.contributor.authorBuehler, Jason
dc.contributor.authorGoodrum, Felicia
dc.contributor.authorNelson, Jay A
dc.date.accessioned2020-01-28T19:49:43Z
dc.date.available2020-01-28T19:49:43Z
dc.date.issued2019-11-14
dc.identifier.citationMikell I, Crawford LB, Hancock MH, Mitchell J, Buehler J, Goodrum F, et al. (2019) HCMV miR-US22 down-regulation of EGR-1 regulates CD34+ hematopoietic progenitor cell proliferation and viral reactivation. PLoS Pathog 15(11): e1007854. https://doi.org/10.1371/journal. ppat.1007854en_US
dc.identifier.issn1553-7366
dc.identifier.pmid31725809
dc.identifier.doi10.1371/journal.ppat.1007854
dc.identifier.urihttp://hdl.handle.net/10150/636745
dc.description.abstractReactivation of latent Human Cytomegalovirus (HCMV) in CD34(+) hematopoietic progenitor cells (HPCs) is closely linked to hematopoiesis. Viral latency requires maintenance of the progenitor cell quiescence, while reactivation initiates following mobilization of HPCs to the periphery and differentiation into CD14(+) macrophages. Early growth response gene 1 (EGR-1) is a transcription factor activated by Epidermal growth factor receptor (EGFR) signaling that is essential for the maintenance of CD34(+) HPC self-renewal in the bone marrow niche. Down-regulation of EGR-1 results in mobilization and differentiation of CD34(+) HPC from the bone marrow to the periphery. In the current study we demonstrate that the transcription factor EGR-1 is directly targeted for down-regulation by HCMV miR-US22 that results in decreased proliferation of CD34(+) HPCs and a decrease in total hematopoietic colony formation. We also show that an HCMV miR-US22 mutant fails to reactivate in CD34(+) HPCs, indicating that expression of EGR-1 inhibits viral reactivation. Since EGR-1 promotes CD34(+) HPC self-renewal in the bone marrow niche, HCMV miR-US22 down-regulation of EGR-1 is a necessary step to block HPC self-renewal and proliferation to induce a cellular differentiation pathway necessary to promote reactivation of virus.en_US
dc.description.sponsorshipNational Institute of Allergy and Infectious DiseasesUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID) [AI21640, P01AI127335, R01 AI079059]; American Cancer Society Post-Doctoral Research Fellowship [129842-PF-16-212-01-TBE]en_US
dc.language.isoenen_US
dc.publisherPUBLIC LIBRARY SCIENCEen_US
dc.rightsCopyright © 2019 Mikell et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.titleHCMV miR-US22 down-regulation of EGR-1 regulates CD34+ hematopoietic progenitor cell proliferation and viral reactivationen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Dept Immunobiol, BIO5 Insten_US
dc.identifier.journalPLOS PATHOGENSen_US
dc.description.noteOpen access journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitlePLoS pathogens
refterms.dateFOA2020-01-28T19:49:43Z


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