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    Human stroma and epithelium co-culture in a microfluidic model of a human prostate gland

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    Author
    Jiang, L
    Ivich, F
    Tahsin, S
    Tran, M
    Frank, S B
    Miranti, C K
    Zohar, Y
    Affiliation
    Univ Arizona, Dept Aerosp & Mech Engn
    Univ Arizona, Dept Biomed Engn
    Univ Arizona, Dept Cellular & Mol Med
    Univ Arizona, Arizona Canc Ctr
    Issue Date
    2019-11-20
    
    Metadata
    Show full item record
    Publisher
    AMER INST PHYSICS
    Citation
    Jiang, L., Ivich, F., Tahsin, S., Tran, M., Frank, S. B., Miranti, C. K., & Zohar, Y. (2019). Human stroma and epithelium co-culture in a microfluidic model of a human prostate gland. Biomicrofluidics, 13(6), 064116.
    Journal
    BIOMICROFLUIDICS
    Rights
    Copyright © 2019 Author(s). Published under license by AIP Publishing.
    Collection Information
    This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
    Abstract
    The prostate is a walnut-sized gland that surrounds the urethra of males at the base of the bladder comprising a muscular portion, which controls the release of urine, and a glandular portion, which secretes fluids that nourish and protect sperms. Here, we report the development of a microfluidic-based model of a human prostate gland. The polydimethylsiloxane (PDMS) microfluidic device, consisting of two stacked microchannels separated by a polyester porous membrane, enables long-term in vitro cocultivation of human epithelial and stromal cells. The porous separation membrane provides an anchoring scaffold for long-term culturing of the two cell types on its opposite surfaces allowing paracrine signaling but not cell crossing between the two channels. The microfluidic device is transparent enabling high resolution bright-field and fluorescence imaging. Within this coculture model of a human epithelium/stroma interface, we simulated the functional development of the in vivo human prostate gland. We observed the successful differentiation of basal epithelial cells into luminal secretory cells determined biochemically by immunostaining with known differentiation biomarkers, particularly androgen receptor expression. We also observed morphological changes where glandlike mounds appeared with relatively empty centers reminiscent of prostatic glandular acini structures. This prostate-on-a-chip will facilitate the direct evaluation of paracrine and endocrine cross talk between these two cell types as well as studies associated with normal vs disease-related events such as prostate cancer.
    Note
    12 month embargo; published online: 20 November 2019
    ISSN
    1932-1058
    PubMed ID
    31768202
    DOI
    10.1063/1.5126714
    Version
    Final published version
    Sponsors
    University of Arizona
    ae974a485f413a2113503eed53cd6c53
    10.1063/1.5126714
    Scopus Count
    Collections
    UA Faculty Publications

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