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dc.contributor.authorKalani, M. Yashar S.
dc.contributor.authorAlsop, Eric
dc.contributor.authorMeechoovet, Bessie
dc.contributor.authorBeecroft, Taylor
dc.contributor.authorAgrawal, Komal
dc.contributor.authorWhitsett, Timothy G.
dc.contributor.authorHuentelman, Matthew J.
dc.contributor.authorSpetzler, Robert F.
dc.contributor.authorNakaji, Peter
dc.contributor.authorKim, Seungchan
dc.contributor.authorVan Keuren-Jensen, Kendall
dc.date.accessioned2020-02-10T20:15:20Z
dc.date.available2020-02-10T20:15:20Z
dc.date.issued2020-01-24
dc.identifier.citationM. Yashar S. Kalani, Eric Alsop, Bessie Meechoovet, Taylor Beecroft, Komal Agrawal, Timothy G. Whitsett, Matthew J. Huentelman, Robert F. Spetzler, Peter Nakaji, Seungchan Kim & Kendall Van Keuren-Jensen (2020) Extracellular microRNAs in blood differentiate between ischaemic and haemorrhagic stroke subtypes, Journal of Extracellular Vesicles, 9:1, 1713540, DOI: 10.1080/20013078.2020.1713540en_US
dc.identifier.issn2001-3078
dc.identifier.doi10.1080/20013078.2020.1713540
dc.identifier.urihttp://hdl.handle.net/10150/636993
dc.description.abstractRapid identification of patients suffering from cerebral ischaemia, while excluding intracerebral haemorrhage, can assist with patient triage and expand patient access to chemical and mechanical revascularization. We sought to identify blood-based, extracellular microRNAs 15 (ex-miRNAs) derived from extracellular vesicles associated with major stroke subtypes using clinical samples from subjects with spontaneous intraparenchymal haemorrhage (IPH), aneurysmal subarachnoid haemorrhage (SAH) and ischaemic stroke due to cerebral vessel occlusion. We collected blood from patients presenting with IPH (n = 19), SAH (n = 17) and ischaemic stroke (n = 21). We isolated extracellular vesicles from plasma, extracted RNA cargo, 20 sequenced the small RNAs and performed bioinformatic analyses to identify ex-miRNA biomarkers predictive of the stroke subtypes. Sixty-seven miRNAs were significantly variant across the stroke subtypes. A subset of exmiRNAs differed between haemorrhagic and ischaemic strokes, and LASSO analysis could distinguish SAH from the other subtypes with an accuracy of 0.972 +/- 0.002. Further analyses predicted 25 miRNA classifiers that stratify IPH from ischaemic stroke with an accuracy of 0.811 +/- 0.004 and distinguish haemorrhagic from ischaemic stroke with an accuracy of 0.813 +/- 0.003. Blood-based, ex-miRNAs have predictive value, and could be capable of distinguishing between major stroke subtypes with refinement and validation. Such a biomarker could one day aid in the triage of patients to expand the pool eligible for effective treatment.en_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.rightsCopyright © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectIntraparenchymal haemorrhage (IPH)en_US
dc.subjectsubarachnoid haemorrhage (SAH)en_US
dc.subjectischaemic strokeen_US
dc.subjectlarge vessel occlusion (LVO)en_US
dc.subjectextracellular microRNA (ex-miRNA)en_US
dc.subjectbiomarkeren_US
dc.titleExtracellular microRNAs in blood differentiate between ischaemic and haemorrhagic stroke subtypesen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Coll Meden_US
dc.identifier.journalJOURNAL OF EXTRACELLULAR VESICLESen_US
dc.description.noteOpen access journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.identifier.pii10.1080/20013078.2020.1713540
dc.source.journaltitleJournal of Extracellular Vesicles
dc.source.volume9
dc.source.issue1
dc.source.beginpage1713540
refterms.dateFOA2020-02-10T20:15:21Z


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Copyright © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as Copyright © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.