THE ROLE OF PHYSALIN F IN REVERSING EXPERIMENTAL NEUROPATHIC PAIN
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractChronic pain continues to be an insurmountable issue in recent times, particularly due to the lack of adequate knowledge as well as the unavailability of appropriate therapeutics that target it. The increasingly common prescription of opioids as medication to treat chronic pain has ultimately contributed to a national opioid epidemic. In response to this, several strides have been made by the Center for Disease Control and Prevention (CDC) to revise guidelines pertaining to opioid prescription for chronic pain. This project specifically focuses on identifying the mechanism of action of Physalin F, a natural compound isolated from the Physalis acutifolia (family: Solanaceae) herb, and previously demonstrated to exhibit antinociceptive effects in models of inflammatory pain, consistent with earlier reports of its anti-inflammatory and immunomodulatory activities. Through the use of calcium imaging, it was revealed that Physalin F had a significant inhibitory effect on voltage-gated calcium channels in dorsal root ganglion. In order to replicate these findings in-vivo, Physalin F was found to reverse mechanical allodynia in both paclitaxel-induced and spinal nerve ligation-induced neuropathic pain models, further elucidating its antinociceptive behavior. Experimentation results provided a better scope on Physalin F mechanism of action as well as grounds for further investigation.
Degree ProgramHonors College