• Login
    View Item 
    •   Home
    • UA Faculty Research
    • UA Faculty Publications
    • View Item
    •   Home
    • UA Faculty Research
    • UA Faculty Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UA Campus RepositoryCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournal

    My Account

    LoginRegister

    About

    AboutUA Faculty PublicationsUA DissertationsUA Master's ThesesUA Honors ThesesUA PressUA YearbooksUA CatalogsUA Libraries

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Environmentally relevant exposure to dibutyl phthalate disrupts DNA damage repair gene expression in the mouse ovary

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Liu&Craig2019_AuthorFinalVersi ...
    Size:
    1.246Mb
    Format:
    PDF
    Description:
    Final Accepted Manuscript
    Download
    Author
    Liu, Xiaosong
    Craig, Zelieann R
    Affiliation
    Univ Arizona, BIO5 Inst
    Univ Arizona, Sch Anim & Comparat Biomed Sci, Shantz Room
    Issue Date
    2019-10-04
    Keywords
    atresia
    cell cycle
    endocrine disruptors
    environmental contaminants and toxicants
    follicle
    follicle development
    follicle maturation
    gene expression
    ovary
    phthalate
    rodents
    toxicology
    Show allShow less
    
    Metadata
    Show full item record
    Publisher
    OXFORD UNIV PRESS INC
    Citation
    Xiaosong Liu, Zelieann R Craig, Environmentally relevant exposure to dibutyl phthalate disrupts DNA damage repair gene expression in the mouse ovary, Biology of Reproduction, Volume 101, Issue 4, October 2019, Pages 854–867, https://doi.org/10.1093/biolre/ioz122
    Journal
    BIOLOGY OF REPRODUCTION
    Rights
    Copyright © The Author(s) 2019. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved.
    Collection Information
    This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
    Abstract
    Phthalates have a history of reproductive toxicity in animal models and associations with adverse reproductive outcomes in women. Human exposure to dibutyl phthalate (DBP) occurs via consumer products (7-10 mu g/kg/day) and medications (1-233 mu g/kg/day). Most DBP toxicity studies have focused on high supraphysiological exposure levels; thus, very little is known about exposures occurring at environmentally relevant levels. CD-1 female mice (80 days old) were treated with tocopherol-stripped corn oil (vehicle control) or DBP dissolved in oil at environmentally relevant (10 and 100 mu g/kg/day) or higher (1000 mu g/kg/day) levels for 30 days to evaluate effects on DNA damage response (DDR) pathway genes and folliculogenesis. DBP exposure caused dose-dependent effects on folliculogenesis and gene expression. Specifically, animals exposed to the high dose of DBP had more atretic follicles in their ovaries, while in those treated with environmentally relevant doses, follicle numbers were no different from vehicle-treated controls. DBP exposure significantly reduced the expression of DDR genes including those involved in homologous recombination (Atm, Brca1, Mre11a, Rad50), mismatch repair (Msh3, Msh6), and nucleotide excision repair (Xpc, Pcna) in a dose-specific manner. Interestingly, staining for the DNA damage marker, gamma H2AX, was similar between treatments. DBP exposure did not result in differential DNA methylation in the Brca1 promoter but significantly reduced transcript levels for the maintenance DNA methyltransferase, Dnmt1, in the ovary. Collectively, these findings show that oral exposure to environmentally relevant levels of DBP for 30 days does not significantly impact folliculogenesis in adult mice but leads to aberrant ovarian expression of DDR genes. Summary Sentence Exposure to human relevant doses of dibutyl phthalate results in significant disruption of DNA damage repair gene expression in the mouse ovary.
    Note
    12 month embargo; published online: 4 October 2019
    ISSN
    0006-3363
    PubMed ID
    31318015
    DOI
    10.1093/biolre/ioz122
    Version
    Final accepted manuscript
    ae974a485f413a2113503eed53cd6c53
    10.1093/biolre/ioz122
    Scopus Count
    Collections
    UA Faculty Publications

    entitlement

    Related articles

    • Environmentally relevant mixtures of phthalates and phthalate metabolites differentially alter the cell cycle and apoptosis in mouse neonatal ovaries†.
    • Authors: Warner GR, Meling DD, De La Torre KM, Wang K, Flaws JA
    • Issue date: 2021 Apr 1
    • Short term exposure to di-n-butyl phthalate (DBP) disrupts ovarian function in young CD-1 mice.
    • Authors: Sen N, Liu X, Craig ZR
    • Issue date: 2015 Jun
    • Effects of in vitro exposure to dibutyl phthalate, mono-butyl phthalate, and acetyl tributyl citrate on ovarian antral follicle growth and viability.
    • Authors: Rasmussen LM, Sen N, Vera JC, Liu X, Craig ZR
    • Issue date: 2017 May 1
    • Daily exposure to Di(2-ethylhexyl) phthalate alters estrous cyclicity and accelerates primordial follicle recruitment potentially via dysregulation of the phosphatidylinositol 3-kinase signaling pathway in adult mice.
    • Authors: Hannon PR, Peretz J, Flaws JA
    • Issue date: 2014 Jun
    • Di-n-butyl phthalate disrupts the expression of genes involved in cell cycle and apoptotic pathways in mouse ovarian antral follicles.
    • Authors: Craig ZR, Hannon PR, Wang W, Ziv-Gal A, Flaws JA
    • Issue date: 2013 Jan
    The University of Arizona Libraries | 1510 E. University Blvd. | Tucson, AZ 85721-0055
    Tel 520-621-6442 | repository@u.library.arizona.edu
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.