Rapid, CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp
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Univ Arizona, Sch Anim & Comparat Biomed Sci, Aquaculture Pathol LabIssue Date
2019-12-23Metadata
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Sullivan, T.J., Dhar, A.K., Cruz-Flores, R. et al. Rapid, CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp. Sci Rep 9, 19702 (2019). https://doi.org/10.1038/s41598-019-56170-yJournal
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Copyright © The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Rapid, sensitive, point-of-care diagnostics are critical for managing infectious diseases. Here we adapt the CRISPR-based SHERLOCK method to develop a rapid, accurate, single copy detection assay for White Spot Syndrome Virus, the most devastating virus impacting global shrimp aquaculture. Further, we combine paper matrix nucleic acid extraction and lateral flow colorimetric reporting to create a fully field-deployable, next-generation diagnostic with potential to transform veterinary pathology, disease ecology, and animal production.Note
Open access journalISSN
2045-2322PubMed ID
31873150Version
Final published versionScopus Count
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Except where otherwise noted, this item's license is described as Copyright © The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License.