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    Long-read sequencing reveals genomic structural variations that underlie creation of quality protein maize

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    Author
    Li, Changsheng
    Xiang, Xiaoli
    Huang, Yongcai
    Zhou, Yong
    An, Dong
    Dong, Jiaqiang
    Zhao, Chenxi
    Liu, Hongjun
    Li, Yubin
    Wang, Qiong
    Du, Chunguang
    Messing, Joachim
    Larkins, Brian A
    Wu, Yongrui
    Wang, Wenqin
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    Affiliation
    Univ Arizona, Sch Plant Sci
    Issue Date
    2020-01-07
    
    Metadata
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    Publisher
    NATURE PUBLISHING GROUP
    Citation
    Li, C., Xiang, X., Huang, Y. et al. Long-read sequencing reveals genomic structural variations that underlie creation of quality protein maize. Nat Commun 11, 17 (2020). https://doi.org/10.1038/s41467-019-14023-2
    Journal
    NATURE COMMUNICATIONS
    Rights
    Copyright © The Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.
    Collection Information
    This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
    Abstract
    Mutation of o2 doubles maize endosperm lysine content, but it causes an inferior kernel phenotype. Developing quality protein maize (QPM) by introgressing o2 modifiers (Mo2s) into the o2 mutant benefits millions of people in developing countries where maize is a primary protein source. Here, we report genome sequence and annotation of a South African QPM line K0326Y, which is assembled from single-molecule, real-time shotgun sequencing reads collinear with an optical map. We achieve a N50 contig length of 7.7 million bases (Mb) directly from long-read assembly, compared to those of 1.04 Mb for B73 and 1.48 Mb for Mo17. To characterize Mo2s, we map QTLs to chromosomes 1, 6, 7, and 9 using an F2 population derived from crossing K0326Y and W64Ao2. RNA-seq analysis of QPM and o2 endosperms reveals a group of differentially expressed genes that coincide with Mo2 QTLs, suggesting a potential role in vitreous endosperm formation.
    Note
    Open access journal
    ISSN
    2041-1723
    PubMed ID
    31911615
    DOI
    10.1038/s41467-019-14023-2
    Version
    Final published version
    ae974a485f413a2113503eed53cd6c53
    10.1038/s41467-019-14023-2
    Scopus Count
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    UA Faculty Publications

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