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dc.contributor.authorLi, Changsheng
dc.contributor.authorXiang, Xiaoli
dc.contributor.authorHuang, Yongcai
dc.contributor.authorZhou, Yong
dc.contributor.authorAn, Dong
dc.contributor.authorDong, Jiaqiang
dc.contributor.authorZhao, Chenxi
dc.contributor.authorLiu, Hongjun
dc.contributor.authorLi, Yubin
dc.contributor.authorWang, Qiong
dc.contributor.authorDu, Chunguang
dc.contributor.authorMessing, Joachim
dc.contributor.authorLarkins, Brian A
dc.contributor.authorWu, Yongrui
dc.contributor.authorWang, Wenqin
dc.date.accessioned2020-03-18T20:56:35Z
dc.date.available2020-03-18T20:56:35Z
dc.date.issued2020-01-07
dc.identifier.citationLi, C., Xiang, X., Huang, Y. et al. Long-read sequencing reveals genomic structural variations that underlie creation of quality protein maize. Nat Commun 11, 17 (2020). https://doi.org/10.1038/s41467-019-14023-2en_US
dc.identifier.issn2041-1723
dc.identifier.pmid31911615
dc.identifier.doi10.1038/s41467-019-14023-2
dc.identifier.urihttp://hdl.handle.net/10150/637742
dc.description.abstractMutation of o2 doubles maize endosperm lysine content, but it causes an inferior kernel phenotype. Developing quality protein maize (QPM) by introgressing o2 modifiers (Mo2s) into the o2 mutant benefits millions of people in developing countries where maize is a primary protein source. Here, we report genome sequence and annotation of a South African QPM line K0326Y, which is assembled from single-molecule, real-time shotgun sequencing reads collinear with an optical map. We achieve a N50 contig length of 7.7 million bases (Mb) directly from long-read assembly, compared to those of 1.04 Mb for B73 and 1.48 Mb for Mo17. To characterize Mo2s, we map QTLs to chromosomes 1, 6, 7, and 9 using an F2 population derived from crossing K0326Y and W64Ao2. RNA-seq analysis of QPM and o2 endosperms reveals a group of differentially expressed genes that coincide with Mo2 QTLs, suggesting a potential role in vitreous endosperm formation.en_US
dc.language.isoenen_US
dc.publisherNATURE PUBLISHING GROUPen_US
dc.rightsCopyright © The Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.en_US
dc.titleLong-read sequencing reveals genomic structural variations that underlie creation of quality protein maizeen_US
dc.typeArticleen_US
dc.contributor.departmentUniv Arizona, Sch Plant Scien_US
dc.identifier.journalNATURE COMMUNICATIONSen_US
dc.description.noteOpen access journalen_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal published versionen_US
dc.source.journaltitleNature communications
dc.source.volume11
dc.source.issue1
dc.source.beginpage17
dc.source.endpage
refterms.dateFOA2020-03-18T20:56:35Z
dc.source.countryEngland


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