Long-read sequencing reveals genomic structural variations that underlie creation of quality protein maize
dc.contributor.author | Li, Changsheng | |
dc.contributor.author | Xiang, Xiaoli | |
dc.contributor.author | Huang, Yongcai | |
dc.contributor.author | Zhou, Yong | |
dc.contributor.author | An, Dong | |
dc.contributor.author | Dong, Jiaqiang | |
dc.contributor.author | Zhao, Chenxi | |
dc.contributor.author | Liu, Hongjun | |
dc.contributor.author | Li, Yubin | |
dc.contributor.author | Wang, Qiong | |
dc.contributor.author | Du, Chunguang | |
dc.contributor.author | Messing, Joachim | |
dc.contributor.author | Larkins, Brian A | |
dc.contributor.author | Wu, Yongrui | |
dc.contributor.author | Wang, Wenqin | |
dc.date.accessioned | 2020-03-18T20:56:35Z | |
dc.date.available | 2020-03-18T20:56:35Z | |
dc.date.issued | 2020-01-07 | |
dc.identifier.citation | Li, C., Xiang, X., Huang, Y. et al. Long-read sequencing reveals genomic structural variations that underlie creation of quality protein maize. Nat Commun 11, 17 (2020). https://doi.org/10.1038/s41467-019-14023-2 | en_US |
dc.identifier.issn | 2041-1723 | |
dc.identifier.pmid | 31911615 | |
dc.identifier.doi | 10.1038/s41467-019-14023-2 | |
dc.identifier.uri | http://hdl.handle.net/10150/637742 | |
dc.description.abstract | Mutation of o2 doubles maize endosperm lysine content, but it causes an inferior kernel phenotype. Developing quality protein maize (QPM) by introgressing o2 modifiers (Mo2s) into the o2 mutant benefits millions of people in developing countries where maize is a primary protein source. Here, we report genome sequence and annotation of a South African QPM line K0326Y, which is assembled from single-molecule, real-time shotgun sequencing reads collinear with an optical map. We achieve a N50 contig length of 7.7 million bases (Mb) directly from long-read assembly, compared to those of 1.04 Mb for B73 and 1.48 Mb for Mo17. To characterize Mo2s, we map QTLs to chromosomes 1, 6, 7, and 9 using an F2 population derived from crossing K0326Y and W64Ao2. RNA-seq analysis of QPM and o2 endosperms reveals a group of differentially expressed genes that coincide with Mo2 QTLs, suggesting a potential role in vitreous endosperm formation. | en_US |
dc.language.iso | en | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.rights | Copyright © The Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/. | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Long-read sequencing reveals genomic structural variations that underlie creation of quality protein maize | en_US |
dc.type | Article | en_US |
dc.contributor.department | Univ Arizona, Sch Plant Sci | en_US |
dc.identifier.journal | NATURE COMMUNICATIONS | en_US |
dc.description.note | Open access journal | en_US |
dc.description.collectioninformation | This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu. | en_US |
dc.eprint.version | Final published version | en_US |
dc.source.journaltitle | Nature communications | |
dc.source.volume | 11 | |
dc.source.issue | 1 | |
dc.source.beginpage | 17 | |
dc.source.endpage | ||
refterms.dateFOA | 2020-03-18T20:56:35Z | |
dc.source.country | England |