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dc.contributor.authorWoo, Ho-Hyung
dc.contributor.authorChambers, Setsuko K
dc.date.accessioned2020-05-18T21:07:34Z
dc.date.available2020-05-18T21:07:34Z
dc.date.issued2020-03-13
dc.identifier.citationWoo, H. H., & Chambers, S. K. (2020). The alternative spliced 3′-UTR mediated differential secretion of macrophage colony stimulating factor in breast cancer cells. Biochemical and Biophysical Research Communications.en_US
dc.identifier.issn0006-291X
dc.identifier.pmid32178869
dc.identifier.doi10.1016/j.bbrc.2020.03.007
dc.identifier.urihttp://hdl.handle.net/10150/641333
dc.description.abstractCSF-1 mRNA 3'UTR variants (var) are generated from alternative splicing. CSF-1 protein encoded by var-1 mRNA with long 3'UTR derived from exon-10 is rapidly secreted compared to the CSF-1 protein encoded by var-4 mRNA with short 3'UTR derived from exon-9. Secretion kinetics indicates that HuR, which binds the CSF-1 var-1 mRNA, but not var-4 mRNA, accelerates the secretion of CSF-1 protein. HuR over-expression increases the secretion rate of CSF-1 protein. In contrast, silencing of HuR does not have such an effect, suggesting other compensatory mechanisms. Effect of the CSF-1 mRNA variant 3'UTRs on cellular phenotype shows both CSF-1 var-1 or -4 mRNA is involved in the enhanced rates of migration and invasion observed by both in vitro in breast cancer cells. Our study indicates that the alternative splicing of CSF-1 mRNA 3'UTR can regulate differential secretion of CSF-1 protein. (C) 2020 Published by Elsevier Inc.en_US
dc.language.isoenen_US
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEen_US
dc.rights© 2020 Published by Elsevier Inc.en_US
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en_US
dc.subjectCSF-1 differential secretionen_US
dc.subjectHuRen_US
dc.subjectVar-1 long 3′UTRen_US
dc.subjectVar-4 short 3′UTRen_US
dc.titleThe alternative spliced 3'-UTR mediated differential secretion of macrophage colony stimulating factor in breast cancer cells.en_US
dc.typeArticleen_US
dc.identifier.eissn1090-2104
dc.contributor.departmentUniv Arizona, Canc Ctren_US
dc.contributor.departmentUniv Arizona, Dept Obstet & Gynecolen_US
dc.identifier.journalBiochemical and biophysical research communicationsen_US
dc.description.note12 month embargo; available online 13 March 2020en_US
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en_US
dc.eprint.versionFinal accepted manuscripten_US
dc.source.journaltitleBiochemical and biophysical research communications
dc.source.volume525
dc.source.issue4
dc.source.beginpage1004
dc.source.endpage1010
dc.source.countryUnited States


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