Simplified method to quantify valve back-leak uncovers severe mesenteric lymphatic valve dysfunction in mice deficient in connexins 43 and 37
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Final Accepted Manuscript
Author
Castorena-Gonzalez, Jorge ASrinivasan, R Sathish
King, Philip D
Simon, Alexander M
Davis, Michael J
Affiliation
Univ Arizona, Dept PhysiolIssue Date
2020-04-08Keywords
Foxc2Rasa1
back-leak
connexin37
connexin43
lymph transport
lymphatic vessel
servo-null micropressure system
valve function
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WILEYCitation
Castorena‐Gonzalez, J.A., Srinivasan, R.S., King, P.D., Simon, A.M. and Davis, M.J. (2020), Simplified method to quantify valve back‐leak uncovers severe mesenteric lymphatic valve dysfunction in mice deficient in connexins 43 and 37. J Physiol. doi:10.1113/JP279472Journal
JOURNAL OF PHYSIOLOGY-LONDONRights
© 2020 The Authors. The Journal of Physiology © 2020 The Physiological Society.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Lymphatic valve defects are one of the major causes of lymph transport dysfunction; however, there are no accessible methods for quantitatively assessing valve function. This report describes a novel technique for quantifying lymphatic valve back-leak. Postnatal endothelial-specific deletion of connexin 43 (Cx43) in connexin 37 null (Cx37-/- ) mice results in rapid regression of valve leaflets and severe valve dysfunction. This method can also be used for assessing the function of venous and lymphatic valves from various species, including humans.Note
12 month embargo; published online: 8 April 2020ISSN
0022-3751EISSN
1469-7793PubMed ID
32267537DOI
10.1113/JP279472Version
Final accepted manuscriptae974a485f413a2113503eed53cd6c53
10.1113/JP279472
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