Simplified method to quantify valve back-leak uncovers severe mesenteric lymphatic valve dysfunction in mice deficient in connexins 43 and 37
AuthorCastorena-Gonzalez, Jorge A
Srinivasan, R Sathish
King, Philip D
Simon, Alexander M
Davis, Michael J
AffiliationUniv Arizona, Dept Physiol
servo-null micropressure system
MetadataShow full item record
CitationCastorena‐Gonzalez, J.A., Srinivasan, R.S., King, P.D., Simon, A.M. and Davis, M.J. (2020), Simplified method to quantify valve back‐leak uncovers severe mesenteric lymphatic valve dysfunction in mice deficient in connexins 43 and 37. J Physiol. doi:10.1113/JP279472
JournalJOURNAL OF PHYSIOLOGY-LONDON
Rights© 2020 The Authors. The Journal of Physiology © 2020 The Physiological Society.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractLymphatic valve defects are one of the major causes of lymph transport dysfunction; however, there are no accessible methods for quantitatively assessing valve function. This report describes a novel technique for quantifying lymphatic valve back-leak. Postnatal endothelial-specific deletion of connexin 43 (Cx43) in connexin 37 null (Cx37-/- ) mice results in rapid regression of valve leaflets and severe valve dysfunction. This method can also be used for assessing the function of venous and lymphatic valves from various species, including humans.
Note12 month embargo; published online: 8 April 2020
VersionFinal accepted manuscript